VPW: Development of Genomic Libraries for Glycine max: A Genetic Tool (Genetics)

VPW：大豆基因组文库的开发：遗传工具（遗传学）

• 批准号: 9253008
• 负责人: Kathleen Danna
• 金额: $ 8.44万
• 依托单位: Northern Arizona University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1994-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9253008&HistoricalAwards=false
• 关键词: VPW; Development; Genomic; Libraries; Glycine

The investigator proposes to produce a tool critical for further studies of soybeans: a collection of large, cloned segments of DNA (P1 library) from two lines: (1) an elite line (BSR101) which has superior genes at most loci and which is representative of the broader germplasm used for breeding; and (2) one which carries exotic genes, including those for resistance to soybean cyst nematode (SCN), a serious pathogen. Dr. Danna will screen the cloned DNA to identify segments that hybridize to molecular markers tightly linked to the SCN-resistance loci. The cloned segments will be useful for obtaining new markers more tightly linked to these important genes and for eventual isolation of the genes for transfer to other lines of soybean. The P1 libraries produced will be useful in addressing fundamental questions about the soybean, including sequence organization of the genome and its evolutionary history from a wild ancestor species. The long term goals of this project are to contribute to the fundamental understanding of the molecular biology and genetics of soybean and to facilitate the manipulation of soybean germplasm for crop improvement. This project furthers VPW program objectives to provide opportunities for women to advance their careers in science or engineering through research, and to encourage other women to pursue careers in these areas through the investigator's enhanced visibility as a role model on the host campus. The proposed activities which contribute to the second objective include: giving guest lectures on virology; teaching an undergraduate seminar course on "Women in Biology," with both an historical perspective and a focus on contemporary biologists; arranging for guest lecturers, who will also present departmental seminars; organizing an informal discussion group for women in the biology department to provide a framework for "networking," and to provide counseling and mentoring for women students.

研究人员建议开发一种工具， 大豆的进一步研究：一个大型的，克隆的 DNA片段（P1文库）来自两个品系：（1）优良品系 （BSR 101），其在大多数基因座处具有上级基因， 代表用于育种的更广泛的种质;以及 (2)一种携带外来基因， 大豆孢囊线虫（SCN）是一种严重的病原菌。 丹娜医生 将筛选克隆的DNA，以确定与 与SCN抗性基因座紧密连锁的分子标记。 的 克隆的片段将有助于获得新的标记， 与这些重要基因紧密相连，并最终分离出 将基因转移到大豆的其他品系中。 P1图书馆 将有助于解决以下基本问题： 大豆，包括基因组的序列组织， 从野生祖先物种进化而来。 该项目的长期目标是促进 对分子生物学和遗传学的基本理解 大豆和促进大豆的操作 作物改良种质 该项目进一步推动了VPW计划的目标， 妇女在科学领域发展事业的机会， 通过研究工程，并鼓励其他妇女， 追求职业生涯在这些领域通过调查员的增强 作为东道校园的榜样。 拟议 有助于实现第二个目标的活动包括： 病毒学客座讲座;教授本科生研讨会 课程“妇女在生物学”，既有历史的角度来看， 并专注于当代生物学家;安排客人 讲师，谁也将提出部门研讨会;组织 生物系女性非正式讨论小组 为“网络”提供一个框架， 为女学生提供咨询和指导。