Functional Studies of Mutations Affecting a Novel DrosophilaMuscle Protein

影响新型果蝇肌肉蛋白的突变的功能研究

• 批准号: 9253045
• 负责人: Jim Vigoreaux
• 金额: $ 25.6万
• 依托单位: University of Vermont & State Agricultural College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-15 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9253045&HistoricalAwards=false
• 关键词: Functional; Studies; Mutations; Affecting; Novel

The main goal of our research is to understand how muscle functions at the molecular level. The system that we study is the indirect muscle of the fruit fly Drosophila melanogaster. AMong the many advantages that this system offers for molecular studies is the fact that these muscles are expendable for viability and therefore amenable for genetic and molecular-genetic analyses. During the course of our investigation, we identified a novel muscle protein that does not resemble any known protein. We hypothesize that this protein is involved in regulation of muscle contraction. Our goal for this proposal is to identify and characterize mutations that affect the gene encoding this protein. We will then analyze the effect of the mutant protein on the ultrastructural, biochemical and mechanical properties of the muscle. These studies will provide valuable information on the biological function of this protein and on the mechanisms regulating force generation during muscle contraction.

我们研究的主要目标是在分子水平上了解肌肉的功能。我们研究的系统是果蝇的间接肌肉。这个系统为分子研究提供的众多优势之一是，这些肌肉是生存能力所必需的，因此可以进行遗传和分子遗传学分析。在我们的研究过程中，我们发现了一种新的肌肉蛋白质，它与任何已知的蛋白质都不相似。我们假设这种蛋白质参与肌肉收缩的调节。我们这项提议的目标是识别和表征影响编码这种蛋白质的基因的突变。然后我们将分析突变蛋白对肌肉的超微结构、生化和机械性能的影响。这些研究将为该蛋白质的生物学功能和肌肉收缩过程中力量产生的调控机制提供有价值的信息。