Cloning & Functional Studies of Mouse Deafness Mutations

克隆

• 批准号: 7545890
• 负责人: KENNETH R JOHNSON
• 金额: $ 54.13万
• 依托单位: JACKSON LABORATORY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-01-01 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7545890
• 关键词: Anatomy; Animal Model; Auditory system; Behavior; Candidate Disease Gene; Chromosome Mapping; Cloning; Communities; Contracts; DNA Sequence; Deformity; Deletion Mutation; Development; Diagnostic; Ear; Electron Microscopy; Embryo; Engineering; Future; Gene Expression; Gene Mutation; Genes; Goals; Grant; Head; Hearing; Hearing problem; Heritability; Human; Immunohistochemistry; In Situ Hybridization; Inherited; Knock-out; Labyrinth; Lead; Location; Maintenance; Maps; Missense Mutation; Molecular; Monitor; Mus; Mutant Strains Mice; Mutate; Mutation; Names; National Institute on Deafness and Other Communication Disorders; Northern Blotting; Paint; Pathology; Pathology processes; Pathway interactions; Pattern; Physiology; Play; Positioning Attribute; Process; Proteins; Reporter Genes; Research; Resolution; Reverse Transcriptase Polymerase Chain Reaction; Role; Screening procedure; Spatial Distribution; Study models; Time; Transcript; base; deafness; gene function; hearing impairment; improved; insight; loss of function; mRNA Expression; malformation; membranous labyrinth; mouse model; mutant; null mutation; positional cloning; programs

DESCRIPTION (provided by applicant): The mouse is an excellent model for studying human hearing disorders because inner ear anatomy and function are similar in both species. The overall goal of our research program is to discover and characterize new mouse deafness mutations to (1) improve understanding of the molecular mechanisms that underlie the normal hearing process and pathologies that lead to deafness, and (2) provide new mouse models to the scientific community. The genes underlying three new mouse deafness mutations, hurry-scurry (hscy), roundabout (rda), and jitterbug (jbg), were identified by positional cloning. None of the genes have been previously associated with hearing or deafness. The first aim of this renewal application is to determine the functions of these three genes as they relate to the development and maintenance of the auditory system. Inner ears of mutant mice will be examined by electron microscopy for ultrastructural anomalies, and temporal and spatial distributions of transcripts and proteins will be determined by in situ hybridization and immunohistochemistry. The second aim of this application is to identify the gene underlying a fourth new mouse deafness mutation (hyperspin, hspn), which causes a gross malformation of the inner ear. To monitor when and how normal development is disrupted, paint-filled membranous labyrinths of inner ears from mutant embryos will be examined at multiple time points. When the hspn gene is identified, its expression pattern will be assessed along with those of other genes that may act in the same developmental pathway. The third aim of this proposal is to continue our screening program to identify new mouse deafness mutations and determine their inheritance, genetic map locations, and associated inner ear pathologies. Heritability of hearing impairment already has been proven for 28 new mutations, and these will be genetically mapped to a resolution of 5 cM or less. Mutant inner ears will be examined for anatomical abnormalities that lead to deafness and that may provide clues to gene function.

描述（由申请人提供）：小鼠是研究人类听力障碍的优秀模型，因为两种动物的内耳解剖结构和功能相似。我们的研究计划的总体目标是发现和描述新的小鼠耳聋突变，以：(1)提高对正常听力过程和导致耳聋的病理基础的分子机制的理解，(2)为科学界提供新的小鼠模型。通过定位克隆技术鉴定了三种新的小鼠耳聋突变基因，分别是：hurry- scry （hscy）、roundabout （rda）和jitterbug （jbg）。这些基因之前都没有被发现与听力或耳聋有关。这项更新应用的第一个目的是确定这三个基因的功能，因为它们与听觉系统的发育和维持有关。通过电镜检查突变小鼠内耳的超微结构异常，并通过原位杂交和免疫组织化学确定转录本和蛋白质的时空分布。该应用程序的第二个目的是确定导致内耳严重畸形的第四种新的小鼠耳聋突变（超旋，hspn）的基因。为了监测正常发育何时以及如何被破坏，将在多个时间点检查来自突变胚胎的内耳充满油漆的膜迷路。当hspn基因被确定后，其表达模式将与其他可能在相同发育途径中起作用的基因一起被评估。本提案的第三个目标是继续我们的筛选计划，以确定新的小鼠耳聋突变，并确定其遗传，基因图谱位置和相关的内耳病理。听力障碍的遗传性已经被证实有28个新的突变，这些突变将被遗传映射到5厘米或更小的分辨率。突变的内耳将被检查，以寻找导致耳聋的解剖异常，这可能为基因功能提供线索。