NSF Young Investigator

NSF 青年研究员

• 批准号: 9257750
• 负责人: Gerben Zylstra
• 金额: $ 32.05万
• 依托单位: Rutgers University New Brunswick
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9257750&HistoricalAwards=false
• 关键词: NSF; Young; Investigator

Research in this laboratory deals with microorganisms that metabolize xenobiotic compounds, especially aromatic hydrocarbons. New microbial strains that have diverse capability in terms of their ability to degrade different aromatic hydrocarbons will be isolated by various enrichment culture techniques. Emphasis is on obtaining novel strains that have the ability to degrade a wide range of related compounds. The metabolic pathway(s) by which the aromatic hydrocarbon is degraded will be determined by standard biochemical assays and analytical techniques. Unique enzymes showing varied capability will be isolated and characterized. The genes involved in the pathway will be cloned and sequence. Site- directed mutagenesis will be utilized to explore the molecular basis for enzyme structure/function. The regulatory control mechanisms for the various steps of the metabolic pathway will be analyzed at the molecular level. New recombinant strains will be constructed that involve multiple genes from various sources. The goal of these genetically constructed strains is to metabolize as wide a range of compounds as possible and as efficiently as possible. Currently the laboratory is focusing on polycyclic aromatic hydrocarbons, phthalate and phthalate esters, and substituted benzenes (chlorobenzene, toluene, and nitrobenzene). %%% This project on aromatic hydrocarbon degradation is environmentally important in toxic waste removal. Understanding of the complex metabolic pathway for degradation of these compounds will aid in improving biodegradability.

该实验室的研究涉及微生物， 代谢异生化合物，特别是芳香烃。 新的微生物菌株具有不同的能力， 它们降解不同芳烃的能力将 通过各种富集培养技术分离。 重点是 获得具有降解广泛的微生物的能力的新菌株， 相关化合物的范围。 通过代谢途径， 芳烃的降解将通过标准 生物化学测定和分析技术。 独特的酶 显示不同的能力将被分离和表征。 的 将克隆和测序参与该途径的基因。 研究中心- 定向诱变将被用来探索分子 酶结构/功能的基础。 的规管 代谢途径的各个步骤的机制将是 在分子水平上进行分析。 新的重组菌株将 构建了一个包含来自不同来源的多个基因的基因组。 的 这些基因构建菌株的目标是代谢， 尽可能广泛的化合物，并尽可能有效地 可能 目前，该实验室正专注于多环 芳烃、邻苯二甲酸酯和邻苯二甲酸酯，以及 取代苯（氯苯、甲苯和硝基苯）。 %%% 这个芳烃降解项目对环境 对于有毒废物的清除很重要。 理解复杂的 降解这些化合物的代谢途径将有助于 改善生物降解性。