US-China Cooperative Research (Biochem): Mechanism of an anion-translocating ATPase

该奖项支持美国韦恩州立大学巴里·罗森与美国生物膜与膜生物技术国家重点实验室、美国动物研究所刘树森的合作。大肠杆菌阴离子易位atp酶的酶学及结构-功能关系。该项目将重点关注氧阴离子泵的催化亚基，它负责从细胞中挤出亚砷酸盐。Rosen博士的小组将进行蛋白质纯化和诱变，Liu博士的小组将进行快速动力学测量。中国合作者是中国最先进的实验室之一（“重点实验室”）的负责人，该实验室得到了中国政府通过世界银行贷款的大力支持。该实验室拥有美国PI实验室所没有的设备，提供了重要的补充能力。罗森博士在韦恩州立大学的实验室里有刘博士的学生；刘和罗森之间的合作将有助于保持中国归国学生的技能和兴趣，为长期合作提供潜力。本项目由中美合作研究计划资助。

This award supports collaboration between Barry Rosen, Wayne State University, and Liu Shusen, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences on the enzymology and structure-function relationships of an anion- translocating ATPase in E. coli. The project will focus on the catalytic subunit of the oxyanion pump, which is responsible for extruding arsenite from cells. Dr. Rosen's group will perform the protein purification and mutagenesis, and Dr. Liu's group will perform the rapid kinetic measurements. The Chinese collaborator is head of one of China's most advanced laboratories ("key labs") which has been heavily supported by the Chinese government through World Bank loans. The lab has equipment unavailable in the US PI's lab, providing an important complementary capability. Dr. Rosen has students of Dr. Liu working in his lab at Wayne State; collaboration between Liu and Rosen will help keep the skills and interests of the returned Chinese students viable, providing potential for longer term collaboration. This project is supported under the US-China Cooperative Research Program.