US-China Cooperative Research (Biochem): Mechanism of an anion-translocating ATPase

该奖项支持韦恩州立大学的Barry Rosen和动物学研究所生物膜与膜生物技术国家重点实验室刘树森之间的合作，中国科学院对大肠杆菌中一种阴离子转运ATPase的酶学和结构与功能关系的研究。该项目将专注于氧阴离子泵的催化亚单位，该亚单位负责从细胞中挤出亚砷酸盐。罗森博士的团队将进行蛋白质纯化和突变，刘博士的团队将进行快速动力学测量。这位中国合作者是中国最先进的实验室之一(“重点实验室”)的负责人，该实验室得到了中国政府通过世界银行贷款的大力支持。该实验室拥有美国PI实验室所没有的设备，提供了重要的补充能力。罗森博士让刘博士的学生在韦恩州立大学的实验室工作；刘博士和罗森博士之间的合作将有助于保持回国中国学生的技能和兴趣，为更长期的合作提供潜力。该项目得到了美国-中国合作研究计划的支持。

This award supports collaboration between Barry Rosen, Wayne State University, and Liu Shusen, State Key Laboratory of Biomembrane and Membrane Biotechnology, Institute of Zoology, Chinese Academy of Sciences on the enzymology and structure-function relationships of an anion- translocating ATPase in E. coli. The project will focus on the catalytic subunit of the oxyanion pump, which is responsible for extruding arsenite from cells. Dr. Rosen's group will perform the protein purification and mutagenesis, and Dr. Liu's group will perform the rapid kinetic measurements. The Chinese collaborator is head of one of China's most advanced laboratories ("key labs") which has been heavily supported by the Chinese government through World Bank loans. The lab has equipment unavailable in the US PI's lab, providing an important complementary capability. Dr. Rosen has students of Dr. Liu working in his lab at Wayne State; collaboration between Liu and Rosen will help keep the skills and interests of the returned Chinese students viable, providing potential for longer term collaboration. This project is supported under the US-China Cooperative Research Program.