US-China Cooperative Research Programs: Cytosystematics of Cyprinid Fishes

这一建议的“鲤科鱼类的细胞系统学”之间的博士。 John R.金德州农工大学和武汉的李玉成教授 该大学由NSF和中国国家共同赞助 教育委员会（SEDC）。 研究人员建议调查种间变异， 10-14种鲤科鱼类染色体NOR表型 来自美国西部和中国大陆。 他们将 研究种间同源性的NOR轴承染色体， 染色体C-分带、限制性内切酶分带和一种新的 开发的方法，产生什么似乎是类似于G-（或 R-）型带。 的长期目标 项目是评估细胞系统学和进化史， 鲤科鱼类，并评估染色体改变的作用， 在进化史上扮演了重要角色 系统目标 这项研究的目的是检验西北部 美洲鲤科鱼类是（i）单系的，和（ii） 从鲤科动物的亚洲血统中进化而来的 雅罗鱼亚科。 每个物种的数据将包括 所有NOR位点的数量、位置和染色体类型。 C-和 其他带型表型将提供同源性、 具有相同表型的NOR染色体， 涉及NOR位点的染色体重排。 染色体数据 将使用外组比较进行遗传学分析。 其他好处将包括两个知识分子的合并， 世界领先的鱼类细胞系统实验室， 未来在各种项目上的合作。 的 调查人员还将试图促进双方的交流， 各种鱼类的酒精保存标本和冷冻组织 物种

This proposal on "Cytosystematics of Cyprinid Fishes" between Dr. John R. Gold Texas A&M University and Professor Li Yucheng of Wuhan University is jointly sponsored by NSF and the Chinese State Educational Commission (SEDC). The investigators propose to investigate interspecific variations in chromosomal NOR phenotypes among 10-14 species of cyprinid fishes from the western United States and mainland China. They will investigate interspecies homologies of NOR-bearing chromosomes by chromosome C-banding, restriction enzyme banding, and a newly developed method which produces what appear to be analogous to G- (or R-) type bands in higher vertebrates. The long-term goals of the project are to assess the cytosystematics and evolutionary history of cyprinid fishes and to evaluate the role that chromosomal alterations have played in that evolutionary history. The systematic objectives of the research are to test the hypotheses that the western North American cyprinids to be examined are (i) monophyletic, and (ii) derived phylogenetically from an Asian lineage in the cyprinid subfamily Leuciscinae. Data for each species examined will include the number, position(s), and chromosome type(s) of all NOR sites. C- and other banding phenotypes will provide critical tests of homology, of NOR chromosomes with identical phenotypes and also putatively identify chromosomal rearrangements involving NOR sites. The chromosomal data will be analyzed phylogenetically using outgroup comparison. Additional benefits will include the intellectual merger of two of the leading fish cytosystematic laboratories in the world, and the possibility of future collaboration on a variety of projects. The investigators will also attempt to promote an exchange of both alcohol-preserved specimens and frozen tissues from a variety of fish species.