PRF/J: Intraspecific Chloroplast-DNA Variation and Phytogeography in the Pacific Northwest

PRF/J：太平洋西北地区种内叶绿体 DNA 变异和植物地理学

• 批准号: 9303299
• 负责人: Jacqueline Soule
• 金额: --
• 依托单位: Individual Award
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-04-01 至 1997-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9303299&HistoricalAwards=false
• 关键词: PRF; Intraspecific; Chloroplast; DNA; Variation

Many plants exhibit a similar geographic distribution, occurring from southeastern Alaska to northern California. Molecular genetic investigations of three species have revealed a pronounced north-south cytoplasmic discontinuity located south of the Wisconsin glacial maximum. These data implicate glaciation as a possible causal factor in creating the discontinuity and raises the possibility of a link between phytogeography and the genetic architecture of plants in the Pacific Northwest. If so, then other plants displaying similar geographic distributions should also exhibit similar patterns of genetic variation. This study addresses two questions: (a) do similar geographic patterns of chloroplast DNA (cpDNA) variation occur throughout a spectrum of Pacific Northwest plant taxa?, (b) are patterns of variation in cytoplasmic and nuclear markers congruent? Populations of five taxonomically diverse taxa (a gymnosperm, a monocot, and dicots from Dilleniidae, Rosidae, and Asteridae) having different life history features will be examined using cpDNA restriction site analysis, rDNA analysis, and enzyme electrophoresis. From these data, phylogenies will be generated and patterns of genetic variation will be assessed. Further analysis will address biogeographical questions and may help clarify the location and extent of suspected glacial refugia. %%% Our knowledge of the extent of intraspecific cpDNA variation will be enhanced through this investigation. It will also serve to facilitate a broad comparison of patterns and extent of cpDNA variation within species to that observed for nuclear genetic markers, improve knowledge of plant genetic architecture, and establish an important link between the areas of systematics and population genetics.

许多植物表现出类似的地理分布，从阿拉斯加东南部到北加州发生。 对三种物种的分子遗传研究表明，位于威斯康星州冰川最大的南部的明显南北细胞质不连续性。 这些数据暗示冰川是造成不连续性的可能因素，并增加了地理学和太平洋西北植物遗传建筑之间建立联系的可能性。 如果是这样，则表现出相似地理分布的其他植物也应表现出相似的遗传变异模式。 这项研究解决了两个问题：（a）叶绿体DNA（cpDNA）变异的类似地理模式是否发生在整个太平洋西北植物分类群中？（b）胞质和核标志物变化的模式是否一致？ 将使用CPDNA限制性站点分析，rDNA分析和酶电泳的五个分类学分类单元（裸露的单室，单子植物和二核）的人群。 从这些数据中，将产生系统发育，并评估遗传变异的模式。 进一步的分析将解决生物地理问题，并可能有助于阐明可疑的冰川避难所的位置和程度。 %%％通过此研究将增强种内CPDNA变异程度的了解。 它还将促进物种内CPDNA变异的模式和程度的广泛比较与核遗传标记的观察到的cpDNA变化程度，改善对植物遗传结构的了解，并在系统学和种群遗传学领域之间建立重要的联系。