Exploring the intraspecific mechanism determining distribution and phenotypic variation of a tropical newt

在我博士课程的第一年，我完成了几个研究目标。我在越南进行了为期三个月的实地调查，收集了100份DNA样本，确定了53个疣蝾螈（Paramesotriton deloustali）的发生地点。在获得CITES和ABS许可后，这些标本被转移到京都大学。此外，我还测量了182只疣蝾螈的体型，并拍摄了它们的腹侧照片。我还收集了23个地点的水温、pH、EC、O2、NO3、NO2、碳酸盐硬度、一般硬度、NH3/NH4、宽度、深度等11项环境指标。最后，我在四个不同的海拔高度（100m， 700m和1100m）进行了粘土模型实验。为了分析这些数据，我回顾了来自相机陷阱的数据，并比较了越南疣蝾螈在白天和晚上的捕食率。我还准备了50个模板DNA样本用于Mig-seq测序，并对调查期间收集的蝾螈样本进行了线粒体DNA测序。此外，我还对越南疣蝾螈的两个主要群体进行了生态位建模。作为我研究的结果，我提交了两篇稿子发表。第一篇题为“粘土模型揭示越南疣蝾螈（尾科：蝾螈科）昼夜捕食率的差异”，已提交给《当代爬行动物学》（目前正在审查中）。第二份手稿“越南疣蝾螈的人口统计学和生态位动态：历史气候影响”已提交给Plos ONE（目前正在审查中）。

In the first year of my doctoral course, I accomplished several research objectives. I conducted a three-month field survey in Vietnam where I collected 100 DNA samples and identified 53 occurrence localities of warty newts (Paramesotriton deloustali). The specimens were then transferred to Kyoto University with CITES and ABS permissions. Additionally, I measured the body size and took ventral body photos of 182 warty newts. I also collected 11 environmental indicators at 23 locations, such as water temperature, pH, EC, O2, NO3, NO2, Carbonate hardness, general hardness, NH3/NH4, Width, and Depth of the stream. Lastly, I conducted clay model experiments at four different elevation levels (100m, 700m, and 1100m).To analyze the data, I reviewed the data from camera traps and compared the predation rates of Vietnam warty newts during the day and night. I also prepared 50 template DNA samples for Mig-seq sequencing and sequenced mitochondrial DNA for newt samples collected during the survey. Additionally, I run ecological niche modeling for two main groups of the Vietnam warty newt.As a result of my research, I submitted two manuscripts for publication. The first is titled "Clay Model Reveals the Difference in Day and Night Predation Rates on Vietnam Warty Newt (Caudata: Salamandridae)" and has been submitted to Current Herpetology (currently under review). The second manuscript, "Demographic and ecological niche dynamics of the Vietnam warty newt, Paramesotriton deloustali: historical climate influences" has been submitted to Plos ONE (currently under review).