Time-Resolved X-ray Absorption Spectroscopy on Microsec. Timescales

微秒时间分辨 X 射线吸收光谱。

• 批准号: 9303830
• 负责人: Mark Chance
• 金额: $ 2万
• 依托单位: Yeshiva University, Albert Einstein College of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-01 至 1995-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9303830&HistoricalAwards=false
• 关键词: Time; Resolved; ray; Absorption; Spectroscopy

Time-resolved structure determinations are at the forefront of attempts to understand the mechanisms of protein reactions. X-ray absorption spectroscopy (XAS) provides a window on the environment of specific atoms in chemical and biological systems, and is well suited to examining the chemistry of metal atoms in biological milieu. In addition, XAS can be applied to a wide range of sample phases, and can successfully probe the chemical environment of a wide range of atoms in the periodic table (from low Z atoms, like sulfur, to heavy atoms, like third row transition metals). We are developing a system for collecting high quality x-ray absorption spectroscopy data on s and longer timescales. The method can be used for both full spectra accumulation and kinetic spectrophotometry. Experiments using this technology have vast potential, not only for examining metalloproteins, but also for observing the dynamic behavior of a wide range of chemical elements in conjunciton with photo-chemical, rapid mixing, and temperature jump techniques. We have tested the system by examining the structural evolution of the myoglobin-carbon monoxide complex after photolysis at low temperature.

时间分辨结构测定处于最前沿 试图了解蛋白质的机制 反应. X射线吸收光谱（XAS）提供了一种 在化学和生物学中特定原子环境的窗口 生物系统，并且非常适合检查 生物环境中的金属原子化学。 此外，本发明还提供了一种方法， XAS可应用于广泛的样品相，并可 成功探测了大范围的化学环境 元素周期表中的原子（从低Z原子，如 硫，重原子，如第三行过渡金属）。 我们正在开发一种收集高质量X射线的系统 s和更长时间尺度上的吸收光谱数据。 该方法既可用于全谱积累， 和动力学分光光度法。 实验用这个 技术具有巨大潜力，不仅用于检查 金属蛋白，而且还用于观察动态 多种化学元素的结合行为 具有光化学、快速混合和温度跳跃 技术. 我们已经通过检查 肌红蛋白-一氧化碳的结构演变 配合物在低温下光解。