RUI: Phospholipid Metabolism in Tissue Culture Cells and Tetrahymena

RUI：组织培养细胞和四膜虫中的磷脂代谢

• 批准号: 9304195
• 负责人: Joseph Smith
• 金额: $ 22.6万
• 依托单位: University of Massachusetts, Dartmouth
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9304195&HistoricalAwards=false
• 关键词: RUI; Phospholipid; Metabolism; Tissue; Culture

9304195 Smith The goals of this research are: 1) to use the hormone-induced differentiation of 3T3-L1 fibroblasts into adipocytes to examine the role of different phospholipid head-groups in receptor structure and function 2) to use altered Phospholipid composition to study regulation of phospholipid metabolism in animal cells and 3) to continue to develop the use of the ciliate protozoan Tetrahymena thermophila as a model system for the study of eukaryotic phospholipid metabolism and the role of different phospholipids in membrane structure and function. The research will involve undergraduates in laboratory experience useful for a scientific career. %%% The goals of this research are to examine the effect of synthetic analogs of membrane components on the function of receptor proteins which exist embedded within the cell membrane, and to use these compounds to study the phospholipid composition of cellular membranes and its regulation in animal cells. Also, the investigator will continue to develop the use of the ciliated protozoan as a model system for the study of eukaryotic phospholipid metabolism and the role of different phospholipids in membrane structure and function. The research will involve undergraduates in laboratory experience useful for a scientific career ***

9304195 Smith本研究的目标是：1）使用3 T3-L1成纤维细胞向脂肪细胞的诱导分化来检查不同磷脂头基在受体结构和功能中的作用2）使用改变的磷脂组成来研究动物细胞中磷脂代谢的调节和3）继续开发利用纤毛虫原生动物嗜热四膜虫作为研究真核磷脂代谢和不同磷脂在膜结构和功能中的作用的模型系统。这项研究将使本科生参与对科学生涯有用的实验室经验。 本研究的目的是检测膜成分的合成类似物对存在于细胞膜内的受体蛋白功能的影响，并使用这些化合物来研究细胞膜的磷脂组成及其在动物细胞中的调节。 此外，研究人员将继续开发使用的纤毛虫原生动物作为模型系统的研究真核磷脂代谢和不同的磷脂在膜结构和功能的作用。这项研究将使本科生参与对科学生涯有用的实验室经验 *