Synthesis, Structure, and Function of Eukaryotic U14 snRNA

真核 U14 snRNA 的合成、结构和功能

• 批准号: 9304672
• 负责人: E Stuart Maxwell
• 金额: $ 35.44万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-01 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9304672&HistoricalAwards=false
• 关键词: Synthesis; Structure; Function; Eukaryotic; U14

9304672 Maxwell Eukaryotic small nuclear RNAs(snRNAs) play critical roles in the processing of both pre-messenger and pre-ribosomal RNAs. One such nucleolar snRNA designated U14 snRNA is essential for the processing of pre-ribosomal RNA. The evolutionarily conserved ability of fungal, amphibian and mammalian U14 snRNAs to intermolecularly base-pair with 18SrRNA suggests that this hydrogen-bonding interaction is important in U14 snRNA function and ribosomal RNA processing events. We have demonstrated that the mouse U14 snRNA genes are located within introns 5,6 and 8 of the constitutively-expressed cognate hsc70 heat shock gene, a positioning which led to experiments confirming that an intron- processing pathway for U14 snRNA production exists. This is the first example of a eukaryotic pre-mRNA intron which is processed to yield a stable, biologically functional RNA species. We will continue characterizing U14 snRNA synthesis, structure and function while exploring the evolutionary conservation of those parameters. The processing sequences/structures essential for U14 snRNA excision will be determined by several different approaches. We will examine the developmental expression of U14 snRNA/hsc70 pre- mRNA in Xenopus laevis. We will examine the genomic organization of U14 snRNA genes in evolutionarily diverse eukaryotic organisms. %%% RNA is utilized both as a structural molecule for protein synthesis as well as a messenger molecule in the same process. Smaller RNA species have been found to be essential to the correct synthesis of the ribosomal and messenger RNAs and a number of them have been isolated and characterized. U14 snRNA is one such molecule, but one which is unique in that it was found by this laboratory to be located in introns of another gene, the hsc70 heat shock gene. This is the first example of an intron which is processed to yield a stable, biologically functional RNA species. The process of producing the U14 snRNA will be studied more extensiv ely as well as the developmental expression and the evolutionary conservation of this RNA species. ***

9304672麦克斯韦真核小核RNA(SnRNA)在前信使RNA和核糖体前RNA的加工过程中发挥着关键作用。其中一种名为U14的核仁SnRNA对于核糖体前RNA的加工是必不可少的。真菌、两栖动物和哺乳动物U14单链RNA与18SrRNA分子间碱基对的进化保守能力表明，这种氢键相互作用在U14单链RNA功能和核糖体RNA处理事件中是重要的。我们已经证明，小鼠U14短链RNA基因位于结构性表达的同源hsc70热休克基因的内含子5、6和8内含子内，这一定位导致实验证实了产生U14短链RNA的内含子加工途径存在。这是第一个真核细胞前mRNA内含子的例子，它被加工成稳定的、具有生物功能的RNA物种。我们将继续表征U14 SnRNA的合成、结构和功能，同时探索这些参数的进化保守。U14 SnRNA切除所必需的处理序列/结构将通过几种不同的方法来确定。我们将检测U14SnRNA/hsc70 Pre-mRNA在非洲爪哇发育过程中的表达。我们将研究U14单链RNA基因在进化多样性的真核生物中的基因组组织。%RNA既是蛋白质合成的结构分子，又是蛋白质合成过程中的信使分子。已发现较小的RNA物种对于正确合成核糖体和信使RNA是必不可少的，其中一些已经被分离和鉴定。U14SnRNA就是这样一个分子，但它的独特之处在于，本实验室发现它位于另一基因hsc70热休克基因的内含子中。这是第一个内含子被加工以产生稳定的、具有生物功能的RNA物种的例子。U14单链RNA的产生过程以及该RNA物种的发育表达和进化保守性将得到更广泛的研究。***