The Molecular Genetics of the Yeast RAD52 Gene

9304937利文斯顿酵母RAD 52基因在染色体维持中起核心作用。 突变细胞在修复受损DNA、重组染色体和完成减数分裂方面的严重缺陷说明了它的重要性。 蛋白质产品的作用尚未确定。 该项目正在研究RAD 52的遗传学。 这项研究开始于温度敏感等位基因的表征，我们已经分离出，在DNA修复或染色体重组中有缺陷。 然后，这些突变体被用来寻找基因外抑制因子。 这样的抑制子将鉴定其产物与RAD 52的蛋白质产物Rad 52 p相互作用的其他基因。 温度敏感的等位基因，以及能够形成孢子的截短等位基因，接下来用于研究Rad 52 p在减数分裂中的作用。分子研究包括蛋白质-蛋白质相互作用的检查：通过酵母蛋白质的融合文库选择的Rad 52 p多肽之间。 另一项分子研究包括检查温度敏感性和其他重组熟练的RAD 52突变体在体内切除双链断裂的程度。 这项研究涉及对所有生物都很重要的两个过程：基因重组和DNA修复。 ***

9304937 Livingston The yeast RAD52 gene plays a central role in chromosome maintenance. Its importance is illustrated by the severe defects mutant cells have in repairing damaged DNA, recombining chromosomes, and in completing melotic division. The role of the protein product has not been defined. This project is examining the genetics of RAD52. The study begins with the characterization of temperature-sensitive alleles, which we have isolated, having defects in either DNA repair or chromosome recombination. These mutants are then used to find extragenic suppressors. Such suppressors will identify other genes whose products interact with Rad52p, the protein product of RAD52. The temperature-sensitive alleles, as well as a truncation allele which is able to sporulate, are next used to study the role of Rad52p in meiosis. Molecular studies include an examination of protein-protein interactions: between Rad52p polypeptide selected through a fusion library of yeast proteins. Another molecular study includes the examination of the extent with which the temperature-sensitive and other recombination-proficient RAD52 mutants resect double strand breaks in vivo. %%% This research is concerned with two processes important to all organisms: genetic recombination and repair of DNA. ***