RUI: Immunobiologic Studies of a Marsupial

9305401石头这个项目的总体目标是启动对灰色短尾负鼠--有袋类动物--家养单尾负鼠的分子免疫生物学研究。已发现斑点鱼表现出典型的一次免疫反应和非典型的二次反应。其他最近的结果表明，该物种缺乏从免疫球蛋白M到免疫球蛋白G的典型真核生物同型转换。进一步的研究将比较Monodelphis和小鼠的免疫反应，小鼠是一种典型的真兽类生物。在与其他实验室的合作中，将使用分子方法来研究主要组织相容性系统和T细胞受体。这项研究将继续涉及聪明、年轻的本科生。哺乳动物的免疫系统是人类健康背景下许多研究的主题，对小鼠和人的免疫功能也有很多了解。相比之下，人们对其他动物的自我识别和防御的类似机制知之甚少。这个项目的总体目标是描述有袋类动物家养单胞鼠的免疫反应，这种灰色的短尾负鼠。该物种是美国唯一一种实验室适应的有袋类动物。由于有袋类动物大约在1.3亿年前从真兽类动物中分离出来，对它们免疫系统的了解可能有助于揭示哺乳动物免疫系统的起源。研究将在细胞和生化水平上进行，并将启动分子免疫生物学研究。在先前NSF的支持下，已经发现Monodelphis表现出典型的初级免疫反应。进一步的研究将比较Monodelphis和小鼠的免疫反应，小鼠是一种典型的真兽类哺乳动物。在与其他实验室的合作中，将使用分子方法来识别MHC基因，并研究T细胞受体。这项研究将继续涉及聪明、年轻的本科生。***

9305401 Stone The overall objective of this project is to initiate molecular immunobiologic studies of the marsupial, Monodelphis domestica, the gray, short-tailed opossum. It has already been found that Monodelphis exhibits a typical primary immune response but an atypical secondary response. Other recent results suggest that the typical eutherian isotype switch from immunoglobulin M to Immunoglobulin G is lacking in this species. Further studies will compare Monodelphis immune responses with the mouse, a typical eutherian organism. In collaboration with other laboratories, molecular approaches will be used to study the major histocompatibility system and the T cell receptor. This research will continue to involve bright, young undergraduate students. %%% The mammalian immune system is the subject of much research in the context of human health, and much is known about immune function in the mouse and man. In contrast, very little is known about the analogous mechanisms of self recognition and defense in other animals. The overall objective of this project is to characterize the immune response of the marsupial, Monodelphis domestica, the gray, short-tailed opossum. This species is the only laboratory- adapted marsupial in America. Because marsupials separated from eutherian mammals about 130 million years ago, knowledge of their immune system may shed light on the origin of the mammalian immune system. Studies will be carried out at the cellular and biochemical levels and molecular immunobiologic studies will be initiated. With prior NSF support it has been found that Monodelphis exhibits a typical primary immune response. Further studies will compare Monodelphis immune responses with the mouse, a typical eutherian mammal. In collaboration with other laboratories molecular approaches will be used to identify MHC genes, and study the T cell receptor. This research will continue to involve bright, young undergraduate students. ***