Immunogenetic Studies of a Marsupial (Monodelphis Domestica)

在未来一年，这项研究将集中完成 其中一些项目是在以前的支助下开始的。i类 主要组织相容性复合物的膜抗原 有袋类Monodelphis arctica将被分离并表征 使用Western blot程序。 基线免疫研究 将完成回复，并尝试使新生儿耐受 颗粒和可溶性抗原将继续。 的进一步数据 淋巴细胞对有丝分裂原的反应以及 在混合淋巴细胞培养中， 得到了 将继续研究阿尔法的发展， 甲胎蛋白在不同阶段的动物，和抗血清， 将产生甲胎蛋白。 最后，将继续研究 酶多态性和血型的遗传控制 已经定义。 本项目的主要目的是研究 免疫遗传学和生化遗传学系统。另一 目标是让聪明的本科生参与基础教育， 在一个成熟和活跃的研究实验室进行研究。

In the coming year, this research will concentrate on completing several of the projects begun under previous support. The Class I membrane antigens of the Major Histocompatibility Complex of the marsupial Monodelphis domestica will be isolated and characterized using the Western blot procedure. Studies of the baseline immune responses will be completed and attempts to tolerize newborns to particulate and soluble antigens will be continued. Further data on the response of lymphocytes to mitogens and the behavior of Monodelphis lymphocytes in mixed lymphocyte cultures will be obtained. Studies will be continued of the development of alpha- fetoprotein in animals of various stages, and antisera against alpha- fetoprotein will be produced. Finally, studies will be continued of the genetic control of the enzyme polymorphisms and blood groups already defined. The major objective of this project is to study the immunogenetic and biochemical genetic systems of a marsupial. Another objective is to involve bright undergraduate students in basic research in an established and active research laboratory.