Chromatin Diminution, Development, and Genome Evolution

染色质减少、发育和基因组进化

• 批准号: 9306607
• 负责人: Grace Wyngaard
• 金额: $ 5.43万
• 依托单位: James Madison University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-15 至 1995-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9306607&HistoricalAwards=false
• 关键词: Chromatin; Diminution; Development; Genome; Evolution

Large-scale genomic rearrangements including the loss of significant amounts of DNA are known to occur during the development of the organism in several groups of animals and some plants, but the mechanisms for these changes and the functions served are not known. Dr. Wyngaard in a career advancement award will be learning new DNA-based technologies to explore the pattern and process of chromatin diminution and genomic rearrangement in a group of freshwater copepods (planktonic crustaceans) where these phenomena are well known. Probes for particular classes of DNA will be used to test whether DNA excisions involve specialized groups of genes and whether the sequence of losses has functional effects in the development of the organism. %%% Chromosome rearrangements and loss of significant amounts of DNA, the genetic material, characterize groups of ciliated protozoa, crustacean copepods, and nematodes during the development of individual organisms. Dr. Wyngaard in a career advancement award is applying powerful new molecular methods to the study of these phenomena, in particular to determine whether classes of genes are excised during development and whether the sequence of losses has functional effects in growth and maturation. The work will advance understanding of molecular genetics and evolution in animals.

大规模的基因组重排， 已知大量的DNA发生在 生物体在几组动物中的发育， 植物，但这些变化的机制和功能 服务不知道。 Wyngaard博士获得职业发展奖 将学习新的基于DNA的技术， 和染色质减少和基因组重排的过程 一组淡水桡足类（浮游甲壳类），其中这些 现象是众所周知的。 特定类型DNA的探针 将用于测试DNA切除是否涉及专门的 基因组以及缺失序列是否具有功能性 对机体发育的影响。 %%% 染色体重排和大量的 DNA是遗传物质， 原生动物、甲壳类桡足类和线虫 个体有机体。 Wyngaard博士的职业发展 该奖项将强大的新分子方法应用于研究 这些现象，特别是要确定是否类 基因在发育过程中被切除， 损失对生长和成熟具有功能性影响。 工作 将促进对分子遗传学和进化的理解， 动物