Assembly of an E coli Membrane Protein Complex

膜蛋白在细胞内起着多种关键作用， 新陈代谢和生理学。 然而，人们对此知之甚少。 蛋白质插入膜和组装的过程 将这些蛋白质转化为功能结构。 一类 膜蛋白，ATP结合盒（ABC）超家族， 包括细菌结合蛋白依赖性转运蛋白， 酵母STE6和囊性纤维化基因蛋白。这一拟议 研究将检查膜的结构和组装过程， 蛋白质使用ABC转运蛋白参与麦芽糖摄取， 大肠杆菌作为模型系统。 这项工作将利用 已经完成的广泛的遗传特征分析， 编码这些蛋白质的mal基因， 跟踪异源低聚麦芽糖的体内组装 运输复合物，以及现有的基因融合方法 在大肠杆菌 %%% 这项工作将测试这些不同的模型， 复杂的膜蛋白，并在适当的时候，导致 膜蛋白不同或修订模型的建议 折页. 这些研究的结果将有助于 更好地了解体内结构和组装 膜蛋白

Membrane proteins play a variety of critical roles in cellular metabolism and physiology. However, little is known about the process of protein insertion into the membrane and of the assembly of these proteins into functional structures. One class of membrane proteins, the ATP binding cassette (ABC) superfamily, includes, the bacterial binding protein-dependent transporters, the yeast STE6, and the cystic fibrosis gene protein. This proposed study will examine the structure and assembly process for membrane proteins using the ABC transporter involved in maltose uptake in Escherichia coli as a model system. This work will take advantage the extensive genetic characterization that has been done on the mal genes which code for these proteins, of a biochemical assay for tracking the in vivo assembly of the hetero-oligomeric maltose transport complex, and of the gene fusion methodologies available in E. coli. %%% This work will test these different models for the assembly of complex membrane proteins and, when appropriate, lead to the proposal of different or revised models for membrane protein folding. The results from these studies will contribute to a greater understanding of the in vivo structure and assembly of membrane proteins.