Electroenzymatic Synthesis using Oxidoreductase Enzymes

使用氧化还原酶的电酶法合成

• 批准号: 9307836
• 负责人: Dale Drueckhammer
• 金额: $ 19万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-08-01 至 1997-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9307836&HistoricalAwards=false
• 关键词: Electroenzymatic; Synthesis; using; Oxidoreductase; Enzymes

9307836 Drueckhammer The focus of this research is the development of preparative electroenzymatic synthetic methodology. The reactions of flavin containing oxidases and dehydrogenases will be driven electrochemically in the reverse direction for the asymmetric synthesis of amino acids and hydroxy acids. Diffusable mediators (viologen derivatives) will be used to transfer electrons from the cathode to the enzyme. Medium to high molecular weight electron transfer mediators will be prepared for use in a size-exclusion membrane enclosed electroenzymatic reactor. Applications of the technique for the synthesis of D-amino acids, L-alpha-hydroxy acids and D-aldose sugars will be probed. %%% With this award, the Chemistry Division and the Division of Chemical and Thermal Systems are jointly supporting the work of Dr. Dale G. Drueckhammer of the Department of Chemistry at Stanford University. The focus of the work, submitted under the EPRI/NSF Initiative in Electrochemical Synthesis, is on developing technology that combines the power of enzyme catalysis with the utility of electrochemistry to achieve preparative scale electroenzymatic synthesis. The success of the project could presage a new area of biocatalysis for use in the enzymatic synthesis of organic and biological compounds. ***

9307836德鲁克哈默本研究的重点是制备电酶合成方法学的发展。由于氨基酸和羟基酸的不对称合成，含有黄素的氧化物酶和脱氢酶的反应将被反向电化学反应驱动。可扩散的媒介(紫精衍生物)将被用来将电子从阴极转移到酶。将制备中、高分子量电子转移介体，用于尺寸排斥膜封闭式电酶反应器。探讨了D-氨基酸、L-α-羟基酸和D-醛糖合成技术的应用。%有了这个奖项，化学部和化学与热系统部共同支持斯坦福大学化学系的Dale G.德鲁克哈默博士的工作。在EPRI/NSF倡议下提交的这项工作的重点是开发将酶催化的力量与电化学的效用相结合的技术，以实现制备规模的电酶合成。该项目的成功可能预示着生物催化的一个新领域，将用于有机和生物化合物的酶合成。***