Electroenzymatic Synthesis using Oxidoreductase Enzymes

9307836 Drueckhammer本研究的重点是制备性电酶促合成方法学的发展。 含有黄素的氧化酶和脱氢酶的反应将在与氨基酸和羟基酸的不对称合成相反的方向上被电化学驱动。 扩散介质（紫精衍生物）将用于将电子从阴极转移到酶。 将制备中等至高分子量的电子转移介质，用于尺寸排阻膜封闭的电酶反应器。 探讨了该技术在D-氨基酸、L-α-羟基酸和D-醛糖合成中的应用。 凭借这一奖项，化学部和化学与热系统部共同支持Dale G.斯坦福大学化学系的Drueckhammer说。 根据EPRI/NSF电化学合成倡议提交的工作重点是开发将酶催化的力量与电化学的实用性相结合的技术，以实现制备规模的电酶合成。 该项目的成功可能预示着一个新的生物催化领域，用于有机和生物化合物的酶促合成。 ***

9307836 Drueckhammer The focus of this research is the development of preparative electroenzymatic synthetic methodology. The reactions of flavin containing oxidases and dehydrogenases will be driven electrochemically in the reverse direction for the asymmetric synthesis of amino acids and hydroxy acids. Diffusable mediators (viologen derivatives) will be used to transfer electrons from the cathode to the enzyme. Medium to high molecular weight electron transfer mediators will be prepared for use in a size-exclusion membrane enclosed electroenzymatic reactor. Applications of the technique for the synthesis of D-amino acids, L-alpha-hydroxy acids and D-aldose sugars will be probed. %%% With this award, the Chemistry Division and the Division of Chemical and Thermal Systems are jointly supporting the work of Dr. Dale G. Drueckhammer of the Department of Chemistry at Stanford University. The focus of the work, submitted under the EPRI/NSF Initiative in Electrochemical Synthesis, is on developing technology that combines the power of enzyme catalysis with the utility of electrochemistry to achieve preparative scale electroenzymatic synthesis. The success of the project could presage a new area of biocatalysis for use in the enzymatic synthesis of organic and biological compounds. ***