Microscopically Controlled Biomineralization at Genetically Engineered Protein Templates

基因工程蛋白质模板的微观控制生物矿化

• 批准号: 9309360
• 负责人: Patrick Stayton
• 金额: $ 9万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-15 至 1996-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9309360&HistoricalAwards=false
• 关键词: Microscopically; Controlled; Biomineralization; Genetically; Engineered

This project is aimed at generating an experimental protein system designed to simultaneously develop a fundamental materials processing capability, and to develop a fundamental understanding of interfacial protein-inorganic crystal molecular recognition. The key contribution of this work arises from the experimental capability to precisely control the three-dimensional spatial distribution of carboxylate residues on a protein surface. Two proteins, streptavidin and protein G, have been carefully selected for this study. They both have a predominance of beta-sheet and delta-helix secondary structure, and, thus, provide a defined scaffolding on which to manipulate the placement of glutamic and aspartic acid side chains. The resolution and three-dimensional spatial control provided by genetic engineering capabilities cannot be currently matched by other techniques. This work thus represents a unique and powerful addition to the experimental characterization of molecular recognition in biomineralization.

该项目旨在产生一个实验性蛋白质系统，旨在同时开发基本的材料处理能力，建立对界面蛋白-无机晶体分子识别的基本认识。这项工作的关键贡献来自于精确控制蛋白质表面羧酸残基的三维空间分布的实验能力。两种蛋白，链亲和素和蛋白G，已被仔细选择用于本研究。它们都具有β -片和δ -螺旋二级结构的优势，因此，为操纵谷氨酸和天冬氨酸侧链的位置提供了一个明确的支架。基因工程能力提供的分辨率和三维空间控制目前是其他技术无法比拟的。因此，这项工作为生物矿化分子识别的实验表征提供了独特而有力的补充。