Application of Adhesion-Deficient VOC-Degrading Bacteria in the Bioremediation of Contaminated Aquifers

WPCM 2 B P Z 10 cpi #|X =V x 6 X @ 8 ; X @ 佳能LBP-8III（附加） CALB8IAD.PRS X @ 0J zX @ #|X 2 Z B % X F ` 佳能LBP-8III（附加） CALB8IAD.PRS X @ 0 J zX @ cpi 10 cpi cpi脚注16 cpi。 ? x x x， X 6 X @ 8 ; X @ + H H H， S、H 6 X @ ; @ t” 不 } r } r 6e t 不 } r 6h t } r . S u t 一 S } r' I ut R PQV 6e L 2 K 小行星9310770 在 原位 生物补救要求有能力的降解细菌能够穿透土壤或含水层固体到达污染物。 这种方法的主要障碍之一是细菌通常强烈粘附在固体表面并堵塞多孔材料，而不是通过它们。 我们的第一阶段研究证明了分离几种不同细菌菌株的非粘性变体的可行性，这些菌株能够降解一系列常见于地下水和含水层中的氯化有机化学品。 在第一阶段开发的菌株有可能旅行至少一个数量级进一步进入含水层固体比他们的粘合剂亲本菌株。 通过使用三氯乙烯作为模型氯化有机物，我们还证明了这些非粘性变体的降解能力没有改变。 第二阶段的研究将使这项技术的商业化，建立了一些操作参数，使用这些非粘性降解菌株 在 原位 . 除了建立粘附缺陷表型的稳定性，我们将测量这些菌株在不同类型的含水层材料中的渗透和降解效率。 将建造一个试验规模的含水层模型， 在 原位 生物修复系统的基础上使用这些高度专业化的微生物。地下水污染是世界范围内日益严重的问题，开发控制地下水污染的方法极为重要。 解决这个问题的一个方法是继续抽水，并在表面净化水。 由于这是非常昂贵的，正在寻求新的方法来破坏水污染的化学品，通过使用微生物在井，或在含水层，本身。 这些细胞可以消耗污染的化学物质并净化水。 然而，在这个过程中，降解污染化合物的细胞粘附在土壤颗粒上，当它们代谢污染物时，它们生长并最终堵塞水井。 为了解决这个问题，我们开发了某些降解污染物、不粘附或不粘附的细菌菌株。 在目前的研究计划中，我们将确定这些非粘附细胞在地下水净化中是否同样有效，但不会生长成障碍物并堵塞流入井中的水流。*** 0* 0*0

WPCM 2 B P Z Courier 10cpi #| x =V x 6 X @ 8 ; X @ Canon LBP-8III (Additional) CALB8IAD.PRS x @ 0J zX @ #| x 2 Z B % X F ` Canon LBP-8III (Additional) CALB8IAD.PRS x @ 0J zX @Courier 10cpi Courier Footnote 16cpi . ? x x x , x 6 X @ 8 ; X @ + H H H , S , H 6 X @ ; @ t" t } r } r 6e t t } r 6h t } r . s u t a s } r' I ut r PQV 6e L 2 K 9310770 DeFlaun In situ bioremediation requires that competent degradative bacteria be able to penetrate soil or aquifer solids to reach the contamination. One of the major obstacles to this approach is that bacteria often adhere strongly to solid surfaces and plug porous materials rather than pass through them. Our Phase I research demonstrated the feasibility of isolating non adhesive variants of several different bacterial strains that are capable of degrading a suite of chlorinated organic chemicals commonly found as contaminants in groundwater and aquifers. The strains that were developed in Phase I have the potential to travel at least an order of magnitude further into aquifer solids than their adhesive parental strains. By using trichloroethylene as a model chlorinated organic, we also demonstrated that the degradative competence of these non adhesive variants had not been altered. Phase II research will bring this technology to commercialization by establishing a number of operating parameters for the use of these non adhesive degradative strains in situ . In addition to establishing the stability of the adhesion deficient phenot ype, we will measure the penetration and degradative efficiency of these strains in different types of aquifer material. A pilot scale model aquifer will be constructed and used to demonstrate an in situ bioremediation system based on the use of these highly specialized microorganisms. %%% Ground water pollution is a growing problem worldwide and the development of methods to control it are extremely important. One method to confront this problem is to continue to pump water and purify it on the surface. Since this is very expensive, new methods are being sought to destroy water contaminating chemicals by using microorganisms in the well, or in the aquifer, itself. These cells can consume contaminating chemicals and purify the water. However, during the process, the cells that degrade the polluting compounds stick to soil particles, and, as they metabolize pollutants, they grow and eventually plug the well. In an attempt to circumvent this problem, we developed certain strains of pollutant degrading, non sticking, or non adhering, bacteria. In the present research program, we will determine if these non adhering cells work equally well in ground water purification, but without growing into a barrier and plugging the flow of water into the well. *** 0*0*0*