SBIR Phase II: Development of a Bioremediation System for the Degradation of Ozone-Depleting Substances

这个小型企业创新研究二期项目涉及开发一种基于生物催化剂的反应器系统，用于破坏已知会导致臭氧消耗和全球变暖的碳氢化合物。在第一阶段的工作中，母牛分枝杆菌JOB5被选为唯一适合用于设计处理空气排放的生物滴滤器的细菌。第二阶段的研究将尝试：(1)建立氢卤烃氧化的潜在共底物；(2)建立在线气相色谱法连续监测污染物卤代烃；(3)验证了camacae JOB5在实验规模生物滴滤中的可行性，并验证了目标化合物的矿化；(4)确定这些生物过滤装置的最佳进料速率；(5)获得生物滴滤器的长期使用数据；(6)将这些信息用于对建造全尺寸生物滴滤器的成本估算进行分析，并与传统的物理/化学处理技术进行比较，预测资本和运营成本；(7)将这些信息纳入该技术的最终报告和商业化计划。有人建议，与目前的传统空气处理方案相比，开发用于去除和破坏碳氢化合物蒸汽排放的生物反应器系统可能会显著降低成本并节省总体成本。这个小型企业创新研究二期项目涉及开发一种基于生物催化剂的反应器系统，用于破坏导致臭氧消耗和全球变暖的污染物化合物。在第一阶段的工作中，母牛分枝杆菌JOB5被选为最适合用于去除空气中这些污染物的生物滴滤器。第二阶段的研究将试图：(1)找出哪种化合物可以用这种基于微生物的过滤系统从空气中去除；(2)开发在线气相色谱法，连续测量空气中这些污染物的浓度；(3)验证了camacae JOB5在实验规模生物滴滤中的可行性，并验证了过滤装置对目标化合物的破坏作用；(4)确定过滤系统破坏污染物的速率；(5)获得生物滴滤器的长期使用数据；(6)将这些信息用于构建全尺寸生物滴滤的成本分析，并将这种新型生物技术的资本投资和运营成本与传统的物理/化学处理技术进行比较。最后，这些数据将被纳入这项新技术的最终报告和商业化计划。有人建议，与目前的传统空气处理方案相比，生物反应器系统的开发可以显著降低成本并节省总体成本。＊＊＊

Deflaun 9423987 This Small Business Innovation Research Phase II project involves the development of a biocatalyst-based reactor system for the destruction of hydrohalocarbon compounds known to contribute to ozone depletion and global warming. During Phase I work, Mycobacterium vaccae JOB5 was selected as uniquely suited for use in biotrickling filters designed to treat air emission. Phase II research will attempt to: (1) establish potential co-substrates for hydrohalocarbon oxidation; (2) develop an on-line gas chromatographic method for continuous monitoring of pollutant halogenated hydrocarbons; (3) show the feasibility of using M. caccae JOB5 in bench-scale biotrickling filters and verify mineralization of target compounds; (4) establish the optimal feeding rate to these biofiltering devices; (5) obtain data on the long-term use of the biotrickling filter; (6) use this information in cost analyses of estimates for construction of a full-scale biotrickling filter, and in projecting capital and operating costs in comparison with those of conventional physical/chemical treatment technologies; and (7) incorporate this information into a final report and commercialization plan for the technology. It is suggested that the development of a bioreactor system for the removal and destruction of hydrohalocarbon vapor emissions potentially could offer significant cost reductions and overall savings compared to today's conventional air treatment options. %%% This Small Business Innovation Research Phase II project involves the development of a biocatalyst-based reactor system for the destruction of pollutant compounds that contribute to ozone depletion and global warming. During Phase I work, Mycobacterium vaccae JOB5 was selected as best suited for use in biotrickling filters designed to remove these pollutants from the air. Phase II research will attempt to: (1) find out what kind of compounds can be removed from air with this microbe-based filter system; (2) deve lop an on-line gas chromatographic method to continuously measure the concentration of these pollutants in air; (3) show the feasibility of using M. caccae JOB5 in bench-scale biotrickling filters and verify that the filtering device destroys the target compounds; (4) establish the rate at which the filter system can destroy pollutants; (5) obtain data on the long-term use of the biotrickling filter; and (6) use this information in cost analyses for building a full-scale biotrickling filter, and comparing capital investments and operating costs for this novel biotechnology with conventional physical/chemical treatment technologies. Finally, these data will be incorporated into a final report and commercialization plan for this novel technology. It is suggested that the development of a bioreactor system could provide significant cost reductions and overall savings compared to today's conventional air treatment options. ***