Cytoskeletal Systems and Calcium Pulses, Waves, and Gradients in Ooplasmic Segregation and Cytokinesis in the Egg of the Medaka (Oryzias latipes), a Teleost

9316125 Fluck PI和他的学生将继续研究细胞骨架系统和胞质钙脉冲、波和梯度在medaka卵的卵浆运动和细胞分裂中的作用。本研究的目的是：a)描述卵浆分离过程中微管的时空组织；B)确定微管和微管相关马达（运动蛋白）是否参与特定类型细胞器（油滴）向细胞植物极的分离，c)确定人工诱导细胞质钙梯度是否会组织胞浆分离，d)确定钙升高区域和微管组织之间是否存在因果关系，e)确定钙脉冲，波浪或梯度伴随着特定的有丝分裂和细胞动力学事件，以及f)确定在发育中的卵裂沟附近的细胞质钙梯度的消散是否会抑制细胞分裂。Fluck博士和他的学生将继续研究受精卵中结构重排和细胞分裂的机制。medaka鱼的卵为这些研究提供了一个很好的系统。特别是，他们将研究细胞的内部骨架结构（细胞骨架系统）和细胞内钙的脉冲、波和梯度在调节卵子内部结构的运动和卵子分裂成两个子细胞中的作用。这些研究结果将使我们对受精卵早期发育中这两个关键事件的生理和结构基础有重要的新认识。＊＊＊

9316125 Fluck The PI and his students will continue to study the roles of cytoskeletal systems and cytosolic calcium pulses, waves, and gradients in ooplasmic movements and cytokinesis in the medaka egg. The objectives of the research are: a) to describe the spatial and temporal organization of microtubules during ooplasmic segregation; b) to determine whether microtubules and a microtubule-associated motor (kinesin) are involved in the segregation of a specific class of cytoplasmic organelles (oil droplets) toward the vegetal pole of the cell, c) to determine whether the artificial induction of a cytosolic calcium gradient will organize ooplasmic segregation, d) to determine whether there is a causal relationship between zones of elevated calcium and microtubule organization, e) to determine whether calcium pulses, waves, or gradients accompany specific mitotic and cytokinetic events, and f) to determine whether dissipation of cytosolic calcium gradients near the developing cleavage furrow will inhibit cytokinesis. %%% Dr. Fluck and his students will continue to study the mechanisms by which structural rearrangements and cell division take place in fertilized eggs. The egg of the medaka fish provide an excellent system for these studies. In particular they will study the roles of the internal skeletal structures of cells (cytoskeletal systems) and pulses, waves and gradients of intracellular calcium in regulating the movements of the egg's internal structure, and in the division of the egg into two daughter cells. The results of these studies will give us important new insight into the physiological and structural bases of these two critical events in the early development of a fertilized egg. ***