Control of the Partitioning of Photosynthate to Developing Seeds

控制光合产物在发育种子中的分配

• 批准号: 9316737
• 负责人: Roger Spanswick
• 金额: --
• 依托单位: Cornell Univ - State: AWDS MADE PRIOR MAY 2010
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-15 至 1998-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9316737&HistoricalAwards=false
• 关键词: Control; Partitioning; Photosynthate; Developing; Seeds

9316737 Spanswick The long term goal of the project is to evaluate the transport and metabolic steps in the seed portion of the partitioning pathway by estimating their flux control coefficients. This will provide a quantitative assessment of their limitation to the overall flux. Peas will be used as a model system. In this first stage of the project, a steady state 13CO2 labeling system will be constructed for use with a nuclear magnetic resonance (NMR) spectrometer. In addition an NMR probe capable of detecting 13C and 1H resonances will be modified to permit insertion from the top of the NMR machine, to accomodate a developing pea fruit and to perform imaging of 1H associated with sugar molecules using a double pulse technique. The main objective is to achieve a functioning system that combines steady state labeling with non-destructive detection of 13C in the developig fruit by NMR. ***

9316737 Spanswick该项目的长期目标是通过估计其通量控制系数来评估分配途径的种子部分中的运输和代谢步骤。这将对它们对总通量的限制提供定量评估。豌豆将被用作模型系统。在该项目的第一阶段，将构建一个稳态13CO2标记系统，用于核磁共振（NMR）光谱仪。此外，将修改能够检测13 C和1H共振的NMR探针，以允许从NMR机器的顶部插入，以适应发育中的豌豆果实，并使用双脉冲技术对与糖分子相关的1H进行成像。主要目的是实现一个功能系统，结合稳态标记与非破坏性检测的13 C在发展中的水果NMR。***