Control of the Partitioning of Photosynthate to Developing Seeds

该项目的长期目标是通过估算其通量控制系数来评估分配途径种子部分的运输和代谢步骤。这将对它们对总通量的限制进行定量评估。豌豆将被用作示范系统。在项目的第一阶段，将构建一个稳定的13CO2标记系统，用于核磁共振（NMR）光谱仪。此外，能够检测13C和1H共振的核磁共振探针将被修改，以允许从核磁共振机器的顶部插入，以适应正在发育的豌豆果实，并使用双脉冲技术对与糖分子相关的1H进行成像。主要目标是实现一个功能系统，结合稳定状态标记和非破坏性检测13C在发展中的果实通过核磁共振。＊＊＊

9316737 Spanswick The long term goal of the project is to evaluate the transport and metabolic steps in the seed portion of the partitioning pathway by estimating their flux control coefficients. This will provide a quantitative assessment of their limitation to the overall flux. Peas will be used as a model system. In this first stage of the project, a steady state 13CO2 labeling system will be constructed for use with a nuclear magnetic resonance (NMR) spectrometer. In addition an NMR probe capable of detecting 13C and 1H resonances will be modified to permit insertion from the top of the NMR machine, to accomodate a developing pea fruit and to perform imaging of 1H associated with sugar molecules using a double pulse technique. The main objective is to achieve a functioning system that combines steady state labeling with non-destructive detection of 13C in the developig fruit by NMR. ***