The Organic Matrix Associated with Calcium Oxalate Formation in Vitis labrusca

与拉布鲁斯卡草酸钙形成相关的有机基质

• 批准号: 9317366
• 负责人: Mary Webb
• 金额: $ 7万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-02-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9317366&HistoricalAwards=false
• 关键词: Organic; Matrix; Associated; Calcium; Oxalate

9317366 Webbab Calcium oxalate formation is the predominant form of biomineralization in plants. Crystals of calcium oxalate form in higher plants in species-specific anatomical and developmental patterns, indicating that their formation is biologically controlled. Crystals generally form within the vacuole in association with an organic matrix, which accumulates prior to crystal formation and provides cellular control over the crystallization process. The long term goals of the proposed research are to understand what induces particular cells to form crystals, and how the organic matrix controls the crystal development. Because expression of the organic matrix is a key event in crystal initiation and differentiation, the proposed studios focus on the organic matrix associated with crystal formation in Vitis labrusca leaves. In particular, research is aimed in characterizing proteins in membrane enclosures which surround developing crystals. These membrane enclosures form prior to crystal initiation, and are in direct contact with the crystal s during development. In the proposed research polyclonal antibodies will be used that were developed against membrane envelope proteins in rabbits. The specific objectives of the research are: 1) to identify and characterize immunoreactive proteins in the membrane enclosures, and 2) to determine the specificity of antibodies for these proteins and for the membrane envelopes. Antibodies produced against the membrane proteins are used to isolate the individual protein components. The isolated proteins will be subjected to amino-terminal sequencing and their sequence compared with other protein sequences already present in data banks. The specificity of the proteins will be examined by immunoblotting crude protein extracts and solubilized membrane envelope proteins, as well as by immunoelectonmicroscopy. %%% Biomineralization is an important process both in plants and animals. Presently little is known of ho w crystals are initiated in plants, and about their growth. The proposed research deals primarily with fundamental problems of cell differentiation and calcium regulation in plant growth and development. It has also broad relevance outside this area as wll, and will provide information about the mechanisms of biomineralization in plants and other organisms. ***

小行星9317366 草酸钙的形成是植物体内生物矿化的主要形式。 草酸钙晶体在高等植物中以物种特异性的解剖和发育模式形成，表明它们的形成是生物控制的。 晶体通常在与有机基质相关的液泡内形成，有机基质在晶体形成之前积累并提供对结晶过程的细胞控制。 这项研究的长期目标是了解是什么诱导特定细胞形成晶体，以及有机基质如何控制晶体发育。 由于有机基质的表达是晶体起始和分化的关键事件，因此建议的工作室将重点放在与葡萄属labrusca叶片中晶体形成相关的有机基质上。 特别是，研究的目的是表征蛋白质的膜外壳周围的发展晶体。 这些膜包体在晶体起始之前形成，并且在发育期间与晶体直接接触。 在拟议的研究中，将使用针对兔膜包膜蛋白开发的多克隆抗体。 本研究的具体目标是：1）鉴定和表征膜包封物中的免疫反应性蛋白，2）确定抗体对这些蛋白和膜包封物的特异性。 针对膜蛋白产生的抗体用于分离单个蛋白质组分。 将对分离的蛋白质进行氨基末端测序，并将其序列与数据库中已存在的其他蛋白质序列进行比较。蛋白质的特异性将通过免疫印迹粗蛋白提取物和溶解的膜包膜蛋白，以及通过免疫电镜检查。 生物矿化是植物和动物的重要过程。 目前对植物中晶体是如何形成的，以及它们的生长情况还知之甚少。 该研究主要涉及植物生长发育中的细胞分化和钙调节的基本问题。 它也有广泛的相关性以外的这一领域作为wll，并将提供有关的机制，在植物和其他生物体的生物矿化的信息。 ***