Gene Regulation of Dormancy in Avena fatua Embryos

燕麦胚胎休眠的基因调控

• 批准号: 9318055
• 负责人: Michael Foley
• 金额: $ 21万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-03-01 至 1998-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9318055&HistoricalAwards=false
• 关键词: Gene; Regulation; Dormancy; Avena; fatua

9318055 Foley The goal of this project is to elucidate the physiological basis for seed dormancy and afterripening-induced breaking of dormancy. The aim of this project is to investigate the molecular basis for dormancy/breaking dormancy using complementary molecular and genetic approaches. Research on seed dormancy has been approached mainly by studying breaking of dormancy which complicates interpretation by confounding breaking of dormancy with germination. The molecular and genetic approaches described herein are distinct in that they focus primarily on maintenance of dormancy. The hypothesis to be tested states that specific genes or their products which are expressed in dormant embryos maintain seed in the dormant state. The expression of these genes is repressed during afterripening of dormant embryos which facilitates subsequent germination. Using in vivo and in vitro translation procedures, about 10 polypeptides have been identified that occur in dormant but not afterripened embryos soon after imbibition. A dormancy-specific recombinant library has been developed and sixteen putative dormancy-specific positive clones, whose expression is modulated by afterripening, have been selected. These clones will be further characterized as to their general expression and nucleic acid sequence. To complement this approach it is proposed that molecular markers be identified for major genes associated with the dormancy trait and simultaneously evaluate/refine the tentative genetic model explaining dormancy in A. fatua. In addition, to confirm any genetic modelbased on F2 populations derived for bulk segregant analysis and to better understand the inheritance of dormancy it is proposed that advanced-generation, F2-derived lines that vary in their degree of dormancy be developed and genetically evaluated. This molecular and genetic approach should provide significant information toward understanding the physiological basis for this arrested stage of plant development. ***

9318055 Foley本项目的目的是阐明种子休眠和后熟诱导打破休眠的生理基础。 该项目的目的是利用互补的分子和遗传方法研究休眠/打破休眠的分子基础。 对种子休眠的研究主要是通过研究休眠的打破来进行的，这使解释复杂化，因为将休眠的打破与发芽混淆。 本文所述的分子和遗传方法是不同的，因为它们主要集中在休眠的维持上。 有待检验的假说指出，在休眠胚中表达的特定基因或其产物使种子保持休眠状态。 这些基因的表达在休眠胚的后熟过程中受到抑制，这有利于随后的萌发。 使用体内和体外翻译程序，已经确定了约10种多肽，发生在休眠，但不后熟胚胎吸胀后不久。 一个休眠特异性重组文库已经开发和16个推定的休眠特异性阳性克隆，其表达是由后熟调制，已被选中。 将进一步表征这些克隆的一般表达和核酸序列。 为了补充这一方法，建议分子标记鉴定与休眠性状相关的主基因，同时评估/完善解释A. fatua。 此外，确认任何遗传modelbased的F2群体获得的散装分离分析，并更好地了解休眠的遗传，建议先进的一代，F2衍生线，不同程度的休眠开发和遗传评估。 这种分子和遗传学的方法应提供重要的信息，了解植物发育的这一停滞阶段的生理基础。 ***