Indole Photophysics: Implications for Protein Studies

吲哚光物理学：对蛋白质研究的影响

• 批准号: 9405442
• 负责人: Bruce Hudson
• 金额: $ 2.4万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9405442&HistoricalAwards=false
• 关键词: Indole; Photophysics; Implications; Protein; Studies

9405442 Hudson The proposal study is an in-depth investigation of the origins of the nonexponential fluorescence decay behavior of indole and its simple methylated derivatives in fluid polar solutions. This behavior will be contrasted with the dipolar relaxation process that is observed with viscous polar solvents which can be distinguished on the basis of its characteristic red-edge excitation behavior. Hypotheses as to the origin of this behavior will be tested. These included a specific exciplex formation process and electron ejection to solvating defects present in polar solvents. The data obtained with a variety of solvents (including mixed solvents with variable composition), variation of temperature, variable excitation and emission wavelength and for a variety of indole analogs will be analyzed using the Maximum Entropy method. %%% This project is a collaborative effort involving workers at the University of Oregon and Professor Alex Demchenko of the Institute of Biochemistry of Kiev, Ukraine. The funds requested are primarily for support of Dr. Demchenko's effort. The benefits to our research, particularly his development of the red-edge excitation effect as a useful diagnostic method, his knowledge of scientific literature in this field available only in Russian and his familiarity with the Maximum Entropy method of analysis of fluorescence data. ***

9405442哈德森建议的研究是对吲哚及其简单甲基化衍生物在液体极性溶液中的非指数荧光衰减行为的起源进行深入研究。这一行为将与在粘性极性溶剂中观察到的偶极驰豫过程形成对比，后者可以根据其特征的红边激发行为来区分。关于这一行为起源的假设将得到检验。这些包括特殊的激基络合物形成过程和电子喷射到溶剂化存在于极性溶剂中的缺陷。各种溶剂(包括组成不同的混合溶剂)、温度的变化、不同的激发波长和发射波长以及各种吲哚类似物的数据将用最大熵方法进行分析。这个项目是俄勒冈大学的工作人员和乌克兰基辅生物化学研究所的亚历克斯·德姆琴科教授共同参与的。申请的资金主要用于支持德姆琴科博士的努力。对我们研究的好处，特别是他将红边激发效应作为一种有用的诊断方法的发展，他在这一领域的科学文献知识只有俄语版，以及他对荧光数据分析的最大熵方法的熟悉。***