Indole Photophysics: Implications for Protein Studies

9405442哈德逊该提案研究是对吲哚及其简单甲基化衍生物在流体极性溶液中的非指数荧光衰减行为的起源的深入调查。 这种行为将与粘性极性溶剂中观察到的偶极弛豫过程形成对比，粘性极性溶剂可以根据其特征红边激发行为进行区分。 关于这种行为起源的假设将得到检验。 这些包括一个特定的激基复合物形成过程和电子喷射溶剂化缺陷存在于极性溶剂。 将使用最大熵方法分析用各种溶剂（包括具有可变组成的混合溶剂）、温度变化、可变激发和发射波长以及各种吲哚类似物获得的数据。 %这个项目是一个合作项目，涉及俄勒冈州大学的工作人员和乌克兰基辅生物化学研究所的Alex Demchenko教授。 所要求的资金主要用于支持德姆琴科博士的工作。 我们研究的好处，特别是他将红边激发效应发展为一种有用的诊断方法，他对该领域科学文献的了解仅限俄语，以及他对荧光数据分析的最大熵方法的熟悉。 ***

9405442 Hudson The proposal study is an in-depth investigation of the origins of the nonexponential fluorescence decay behavior of indole and its simple methylated derivatives in fluid polar solutions. This behavior will be contrasted with the dipolar relaxation process that is observed with viscous polar solvents which can be distinguished on the basis of its characteristic red-edge excitation behavior. Hypotheses as to the origin of this behavior will be tested. These included a specific exciplex formation process and electron ejection to solvating defects present in polar solvents. The data obtained with a variety of solvents (including mixed solvents with variable composition), variation of temperature, variable excitation and emission wavelength and for a variety of indole analogs will be analyzed using the Maximum Entropy method. %%% This project is a collaborative effort involving workers at the University of Oregon and Professor Alex Demchenko of the Institute of Biochemistry of Kiev, Ukraine. The funds requested are primarily for support of Dr. Demchenko's effort. The benefits to our research, particularly his development of the red-edge excitation effect as a useful diagnostic method, his knowledge of scientific literature in this field available only in Russian and his familiarity with the Maximum Entropy method of analysis of fluorescence data. ***