Oak Tannins, Gypsy Moths, and LdNPV: Anti-Viral Activity Depends on Oxidation and Covalent Binding of Gallotannins to Viral Proteins

9407308外观植物化学单宁在生态相互作用中的高度可变活性导致了对其生态学意义的相当大的争议。这种差异和争议似乎是因为没有认识到氧化在激活这些分子中的重要性。也就是说，植物中的化学物质接受单宁中的电子，这反过来又促进了单宁在昆虫肠道中作为抗病毒药物的作用。在这项研究中，将通过在植物氧化剂存在和不存在的情况下分析单宁的抗病毒活性以及通过在吉普赛飞蛾的模型肠道系统中证明这些被氧化的单宁与病毒蛋白化学结合来证明氧化对单宁作用的重要性来研究电子给予和化学键的重要性。***

9407308 Appel The highly variable activity of plant chemical tannins in ecological interactions has led to considerable controversy about their ecological significance. It appears that this variation and controversy arise from a failure to realize the importance of oxidation in activating these molecules. That is, chemicals in the plant accept electrons from tannins and this in turn facilitates the action of tannins as anti-viral agents in the insect gut. In this study the importance of electron donation and chemical bonding will be investigated by demonstrating the importance of oxidation to tannin action by assaying the antiviral activity of tannins in the presence and absence of plant oxidants and by demonstrating that these oxidized tannins bind chemically to viral proteins in the model gut system of the Gypsy Moth. ***