Videomicroscopy and Microinjection Studies of Maize Meiocytes

玉米性母细胞的视频显微镜和显微注射研究

• 批准号: 9407867
• 负责人: Sue Wick
• 金额: $ 6万
• 依托单位: University of Minnesota-Twin Cities
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-05-15 至 1996-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9407867&HistoricalAwards=false
• 关键词: Videomicroscopy; Microinjection; Studies; Maize; Meiocytes

9407867 Wick This is a Career Advancement Award to support research activities during Dr. Wick's sabbatical. The aim of the project is to develop maize meiocytes as a useful system for studying the dynamics of cytoskeletal elements during meiosis. In collaboration with her sabbatical hosts, the PI will establish a culture system for maize meiocytes that will allow their observation by differential interference microscopy in the living state as the cells undergo meiosis. Specific antibodies will be microinjected, or specific reagents known to disrupt microtubules or actin microfilaments will be applied to the cells, and the effects of these perturbants on meiosis, division plane determination, and cytokinesis in wild-type and male-sterile mutant maize will be assessed. %%% This is a Career Advancement Award to support research activities during Dr. Wick's sabbatical. The process of meiosis, or "reduction division," is critically important to sexual reproduction in eukaryotes, but is very poorly understood. Meiosis is a specialized , complex, multi-step type of cellular division, in which a diploid cell gives rise to a haploid gamete. In this process, the genetic material of the diploid progenitor cell, which is present as pairs of chromosomes one each of which was originally derived from each parental gamete, is very accurately split into two separate but functionally equivalent portions, each portion containing one each of each of the pairs. The ultimate fate of the resulting gamete is sexual union with the corresponding other-sex gamete, to give rise to a new diploid zygote which then develops into a new organism. In this project, the model organism under study is maize (corn), which is not only a potentially useful model system for higher plant meiosis in general, but is in its own right an important subject of study given the agricultural and nutritional significance of hybrid corn. The experiments proposed will provide important insights into the cellular mechanisms whereby the meiotic chromosomes are separated from each other. ***

小行星9407867 这是一个职业发展奖，以支持维克博士休假期间的研究活动。 该项目的目的是开发玉米减数分裂细胞作为一个有用的系统，研究减数分裂过程中的细胞骨架元素的动态。 在与她的休假主机合作，PI将建立一个玉米减数分裂细胞的培养系统，这将允许他们在活状态下观察细胞进行减数分裂的微分干涉显微镜。 将显微注射特异性抗体，或将已知破坏微管或肌动蛋白微丝的特异性试剂应用于细胞，并评估这些干扰剂对野生型和雄性不育突变玉米中减数分裂、分裂平面确定和胞质分裂的影响。 这是一个职业发展奖，以支持维克博士休假期间的研究活动。 减数分裂过程，或称“减数分裂”，对真核生物的有性生殖至关重要，但人们对其了解甚少。 减数分裂是一种特殊的，复杂的，多步骤的细胞分裂类型，其中二倍体细胞产生单倍体配子。 在这个过程中，二倍体祖细胞的遗传物质以成对染色体的形式存在，其中每一对染色体最初来源于每一个亲本配子，二倍体祖细胞的遗传物质被非常准确地分裂成两个独立但功能等同的部分，每个部分含有每一对染色体中的每一个。 配子的最终命运是与相应的异性配子有性结合，产生一个新的二倍体合子，然后发育成一个新的生物体。 在这个项目中，研究的模式生物是玉米（玉米），它不仅是一个潜在的有用的模式系统，一般高等植物减数分裂，但在其本身的权利，一个重要的研究课题，鉴于杂交玉米的农业和营养意义。 提出的实验将提供重要的见解，从而减数分裂染色体彼此分离的细胞机制。 ***