RUI: A Nikon Eclipse TE2000-U Research Inverted Microscope and Microinjection System for Studies of Calcium Signaling in Plants

RUI：用于植物钙信号研究的 Nikon Eclipse TE2000-U 研究倒置显微镜和显微注射系统

• 批准号: 0302330
• 负责人: Edward Tucker
• 金额: $ 8.15万
• 依托单位: CUNY Baruch College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-15 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0302330&HistoricalAwards=false
• 关键词: RUI; Nikon; Eclipse; TE2000; Research

A grant has been awarded to Baruch College under the direction of Dr. Edward Tucker for the acquisition of an inverted microscope and ancillary digital imaging equipment, a micropipette puller, and a micromanipulator for a plant microinjection/calcium imaging facility. Projects will include investigation of the role of calcium in mitosis and as a second messenger in light and hormone signaling. While microinjection and calcium imaging in animals is a routine procedure, in plants these techniques require special technical skills. This facility supported by this grant will be one of a few worldwide where moss cells are microinjected. It will fulfill a demand for a facility where Baruch College moss microinjection experiments can be carried out, and where collaborators from around the world can be trained in moss transformation and microinjection.

在爱德华·塔克博士的指导下，巴鲁克学院获得了一笔赠款，用于购买倒置显微镜和辅助数字成像设备、微量移液器拉具和植物显微注射/钙成像设施的显微操作器。 项目将包括调查钙在有丝分裂中的作用，以及作为光和激素信号中的第二信使。 虽然显微注射和钙成像在动物中是一种常规程序，但在植物中，这些技术需要特殊的技术技能。 该基金支持的设施将是世界上为数不多的显微注射苔藓细胞的设施之一。 它将满足一个设施的需求，在那里可以进行巴鲁克学院苔藓显微注射实验，并在那里来自世界各地的合作者可以在苔藓转化和显微注射培训。