RUI: A Nikon Eclipse TE2000-U Research Inverted Microscope and Microinjection System for Studies of Calcium Signaling in Plants

在爱德华·塔克（Edward Tucker）博士的指导下，巴鲁克学院获得了一笔赠款，用于购买倒置显微镜和辅助数字成像设备、微移液器牵引器和用于植物显微注射/钙成像设备的显微操纵器。项目将包括研究钙在有丝分裂中的作用以及作为光和激素信号传导的第二信使。虽然动物体内的显微注射和钙显像是一种常规程序，但在植物体内，这些技术需要特殊的技术技能。这个由这笔拨款支持的设施将是世界上少数几个进行苔藓细胞微注射的设施之一。它将满足对巴鲁克学院进行苔藓显微注射实验的设施的需求，并为来自世界各地的合作者提供苔藓转化和显微注射方面的培训。

A grant has been awarded to Baruch College under the direction of Dr. Edward Tucker for the acquisition of an inverted microscope and ancillary digital imaging equipment, a micropipette puller, and a micromanipulator for a plant microinjection/calcium imaging facility. Projects will include investigation of the role of calcium in mitosis and as a second messenger in light and hormone signaling. While microinjection and calcium imaging in animals is a routine procedure, in plants these techniques require special technical skills. This facility supported by this grant will be one of a few worldwide where moss cells are microinjected. It will fulfill a demand for a facility where Baruch College moss microinjection experiments can be carried out, and where collaborators from around the world can be trained in moss transformation and microinjection.