Analysis of the Ribonucleases of Arabidopsis thaliana

9408052绿色本研究将探讨模式植物拟南芥的核糖核酸降解酶(RNase)的调控和功能。我们的初步工作导致了拟南芥核糖核酸酶的生化特性和三个核糖核酸酶基因的克隆。为了加强这些方法，已经分离出了几个具有改变RNase模式的拟南芥突变体。在这些研究的基础上，本项目的目标如下：1)产生和利用抗体来研究拟南芥核糖核酸酶的定位和调控；2)通过分析各种遗传杂交的后代来鉴定核糖核酸酶突变体；3)通过突变分析和反义方法来检测拟南芥核糖核酸酶的功能。重点将放在有助于mRNA稳定和/或磷酸盐再动员的核糖核酸酶的鉴定上。这项研究应该为RNase在植物特有的或具有广泛意义的基本过程中的作用提供重要的见解。除了对基础知识的贡献外，这项工作还可以识别其操纵可能导致作物改良的RNAs。***

9408052 Green This research will investigate the regulation and function of the RNA-degrading enzymes (RNases) of the model plant Arabidopsis thaliana. Our initial work has led to the biochemical characterization of the Arabidopsis RNase profile and the cloning of three RNase genes. To augment these approaches, several mutants of Arabidopsis with altered RNase patterns have been isolated. Based on these studies, the objectives of the present project are as follows: 1) Generate and utilize antibodies to study the location and regulation of Arabidopsis RNases; 2) Characterize the RNase mutants by analyzing the progeny of various genetic crosses; 3) Examine the function of Arabidopsis RNases via mutant analysis and antisense approaches. Emphasis will be placed on the identification of RNases that contribute to mRNA stability and/or phosphate remobilization. This research should provide important insight into the roles of RNases in fundamental processes that may be unique to plants or of broad significance. Beyond its contribution to basic knowledge, this work could identify RNases whose manipulation might lead to crop improvement. ***