Analysis of the Ribonucleases of Arabidopsis thaliana

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9408052 Green This research will investigate the regulation and function of the RNA-degrading enzymes (RNases) of the model plant Arabidopsis thaliana. Our initial work has led to the biochemical characterization of the Arabidopsis RNase profile and the cloning of three RNase genes. To augment these approaches, several mutants of Arabidopsis with altered RNase patterns have been isolated. Based on these studies, the objectives of the present project are as follows: 1) Generate and utilize antibodies to study the location and regulation of Arabidopsis RNases; 2) Characterize the RNase mutants by analyzing the progeny of various genetic crosses; 3) Examine the function of Arabidopsis RNases via mutant analysis and antisense approaches. Emphasis will be placed on the identification of RNases that contribute to mRNA stability and/or phosphate remobilization. This research should provide important insight into the roles of RNases in fundamental processes that may be unique to plants or of broad significance. Beyond its contribution to basic knowledge, this work could identify RNases whose manipulation might lead to crop improvement. ***