Analysis of the Ribonucleases of Arabidopsis thaliana

9408052绿色本研究将研究模式植物拟南芥RNA降解酶（RNases）的调控和功能。 我们最初的工作已经导致了拟南芥RNA酶谱的生化表征和三个RNA酶基因的克隆。 为了增强这些方法，已经分离出具有改变的RNA酶模式的拟南芥突变体。 在此基础上，本研究的主要目的如下：1）制备并利用抗体研究拟南芥RNA酶的定位和调控; 2）通过对不同遗传杂交后代的分析，鉴定拟南芥RNA酶突变体; 3）通过突变体分析和反义技术研究拟南芥RNA酶的功能。 重点将放在鉴定RNA酶，有助于mRNA的稳定性和/或磷酸盐再动员。 这项研究应该提供重要的洞察RNases在基本过程中的作用，可能是独特的植物或具有广泛的意义。 除了对基础知识的贡献外，这项工作还可以确定RNA酶，其操纵可能导致作物改良。 ***

9408052 Green This research will investigate the regulation and function of the RNA-degrading enzymes (RNases) of the model plant Arabidopsis thaliana. Our initial work has led to the biochemical characterization of the Arabidopsis RNase profile and the cloning of three RNase genes. To augment these approaches, several mutants of Arabidopsis with altered RNase patterns have been isolated. Based on these studies, the objectives of the present project are as follows: 1) Generate and utilize antibodies to study the location and regulation of Arabidopsis RNases; 2) Characterize the RNase mutants by analyzing the progeny of various genetic crosses; 3) Examine the function of Arabidopsis RNases via mutant analysis and antisense approaches. Emphasis will be placed on the identification of RNases that contribute to mRNA stability and/or phosphate remobilization. This research should provide important insight into the roles of RNases in fundamental processes that may be unique to plants or of broad significance. Beyond its contribution to basic knowledge, this work could identify RNases whose manipulation might lead to crop improvement. ***