Genome-wide Analysis of miRNAs and siRNAs associated with Abiotic Stress in Arabidopsis

拟南芥中与非生物胁迫相关的 miRNA 和 siRNA 的全基因组分析

基本信息

  • 批准号:
    0548569
  • 负责人:
  • 金额:
    $ 56.07万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2006
  • 资助国家:
    美国
  • 起止时间:
    2006-05-15 至 2010-04-30
  • 项目状态:
    已结题

项目摘要

One of the most important discoveries in molecular biology in recent years was that small RNA molecules are very important for regulating genes. Plants have two main types of small RNA, the microRNAs (miRNAs) and the small interfering RNAs (siRNAs), that can be distinguished by how they are made in the cell. These small RNAs range in size from about 21-24 nucleotides, and were missed for many years because they are so tiny. Despite their late discovery, small RNAs are very important in both plants and animals because they function to shut off genes. Small RNAs recognize the target genes they silence by base-pairing interactions. This project will investigate small RNA molecules of the model plant Arabidopsis on a genome-wide scale and investigate the functional significance of those associated with how plants respond to environmental stress. Deep sequencing of a small number of collections made from key small RNA metabolism mutants and plants subjected to stress will result in more than 200,000 sequences per sample. Analysis of the data will provide insight about which small RNAs are miRNAs and which are siRNAs. Assembly of a DNA microarray containing many small RNA sequences will provide the means to test additional mutants and stresses for their regulatory effect on the levels of various small RNAs. These data as well as the incorporation of miRNA/target RNA information into a computer model will lead to new hypotheses about the roles of small RNAs in environmental stress. Many tools to examine these roles are well-established, such as miRNA sensor genes, and overexpression and knockout lines. If time permits, it will be straightforward to begin testing major predictions of some of these hypotheses within the funding period. This project will identify key examples of miRNA involvement in environmental stress and lay the foundation for studying the mechanistic details in the future. Because many Arabidopsis miRNAs are conserved in rice and other plants, this work should lead to the discovery of new functions of general significance.
近年来分子生物学中最重要的发现之一是小RNA分子对调控基因非常重要。植物有两种主要的小RNA,microRNAs(MiRNAs)和小干扰RNAs(SiRNAs),可以通过它们在细胞中的合成方式来区分。这些小RNA的大小从21-24个核苷酸不等,由于它们太小了,多年来一直没有找到。尽管发现较晚,但小RNA在植物和动物中都非常重要,因为它们的功能是关闭基因。小RNA通过碱基配对相互作用识别它们沉默的目标基因。该项目将在全基因组范围内研究模式植物拟南芥的小RNA分子,并调查那些与植物如何响应环境胁迫相关的功能意义。对来自关键的小RNA代谢突变体和遭受胁迫的植物的少量收集的深度测序将导致每个样本超过200,000个序列。对数据的分析将提供关于哪些小RNA是miRNA,哪些是siRNA的洞察力。组装包含许多小RNA序列的DNA微阵列将提供测试额外突变体和应激对各种小RNA水平的调节作用的手段。这些数据以及将miRNA/靶RNA信息整合到计算机模型中将导致关于小RNA在环境压力中的作用的新假设。许多检测这些角色的工具都是成熟的,例如miRNA传感器基因,以及过表达和敲除品系。如果时间允许,在资助期内开始测试其中一些假设的主要预测将是直截了当的。本项目将确定miRNA参与环境胁迫的关键实例,并为未来研究其机制细节奠定基础。由于许多拟南芥miRNAs在水稻和其他植物中是保守的,这项工作应该会导致发现具有普遍意义的新功能。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Pamela Green其他文献

868 HEPATOCELLULAR CARCINOMA INCIDENCE DECLINES AFTER WIDESPREAD INTRODUCTION OF DIRECT-ACTING ANTIVIRALS FOR HEPATITIS C
  • DOI:
    10.1016/s0016-5085(20)31136-7
  • 发表时间:
    2020-05-01
  • 期刊:
  • 影响因子:
  • 作者:
    Lauren Beste;Pamela Green;Kristin Berry;Pamela S. Belperio;George N. Ioannou
  • 通讯作者:
    George N. Ioannou
Self-Reported Everyday Functioning After COVID-19 Infection
COVID-19 感染后自我报告的日常功能
  • DOI:
  • 发表时间:
    2024
  • 期刊:
  • 影响因子:
    13.8
  • 作者:
    Theodore J. Iwashyna;Valerie A Smith;S. Seelye;A. Bohnert;Edward J. Boyko;Denise M. Hynes;George N. Ioannou;Matthew L. Maciejewski;A. O’Hare;E. Viglianti;Theodore Berkowitz;John Pura;James Womer;L. Kamphuis;Max L. Monahan;C. B. Bowling;Andrew Admon;Kathleen Akgun;Stacy Anderson;Mihaela Aslan;David Au;Lisa I Backus;Kristina Bajema;Aaron Baraff;Lisa Batten;Theodore Berkowitz;Taylor Bernstein;Kristin Berry Wyatt;Joseph Bogdan;H. Bosworth;Nathan Boucher;Nicholas Burwick;Aissa Cabrales;J. Cano;Wen Chai;Jason Chen;Kei;Kristina Crothers;Jeffrey Curtis;Marie Davis;Emily Del Monico;A. Dobalian;Jacob Doll;Jason A Dominitz;McKenna Eastment;Vincent Fan;Jacqueline Ferguson;Breanna Floyd;A. Fox;Matthew Goetz;D. Govier;Pamela Green;S. N. Hastings;Katie Hauschildt;Eric J Hawkins;Paul L. Hebert;M. Helfand;A. Hickok;D. Horowitz;Catherine Hough;Elaine Hu;Kevin Ikuta;Barbara Jones;Makoto Jones;L. Kamphuis;Brystana Kaufman;Sara Knight;A. Korpak;Peggy Korpela;Kyle Kumbier;K. Langa;Ryan Laundry;S. Lavin;Yuli Li;Jennifer Linquist;Holly McCready;Martha Michel;Amy Miles;J. Milne;Max L. Monahan;Daniel Morelli;Pradeep Mutalik;Jennifer Naylor;Meike Neiderhausen;S. Newell;Shannon Nugent;Michael Ong;Thomas Osborne;Matthew Peterson;Alexander Peterson;Hallie Prescott;N. Rajeevan;Ashok Reddy;Marylena Rouse;M. Rowneki;Somnath Saha;Sameer Saini;Javeed Shah;T. Shahoumian;Aasma Shaukat;M. Shepherd;Whitney Showalter;Christopher G. Slatore;Nicholas Smith;Battista Smith;Pradeep Suri;Jeremy Sussman;Yumie Takata;Alan Teo;Eva Thomas;Laura Thomas;Anais Tuepker;Zachary P Veigulis;Elizabeth Vig;Kelly Vranas;X. Q. Wang;Katrina Wicks;Kara A Winchell;Edwin S Wong;Chris Woods;Katherine Wysham;Lei Yan;Donna Zulman
  • 通讯作者:
    Donna Zulman
College student perceptions of teaching and learning quality
大学生对教学质量的看法
1037 – Perioperative Mortality and Morbidity Among 8,193 Patients with Cirrhosis Undergoing Emergent Versus Non-Emergent Abdominal Surgery in a National Healthcare System
  • DOI:
    10.1016/s0016-5085(19)40617-3
  • 发表时间:
    2019-05-01
  • 期刊:
  • 影响因子:
  • 作者:
    Kira L. Newman;Kay Johnson;Paul Cornia;Kristin Wyatt;Pamela Green;Peter Wu;George N. Ioannou
  • 通讯作者:
    George N. Ioannou
311 – Non-Emergent Abdominal Surgery in Cirrhotic Patients: A Study of Perioperative Mortality, Morbidity, and Risk Factors in 7,075 Patients in a National Healthcare System
  • DOI:
    10.1016/s0016-5085(19)39945-7
  • 发表时间:
    2019-05-01
  • 期刊:
  • 影响因子:
  • 作者:
    Kira L. Newman;Kay Johnson;Paul Cornia;Kristin Wyatt;Pamela Green;Peter Wu;George N. Ioannou
  • 通讯作者:
    George N. Ioannou

Pamela Green的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Pamela Green', 18)}}的其他基金

Analysis of the Ribonucleases of Arabidopsis thaliana
拟南芥核糖核酸酶分析
  • 批准号:
    1817764
  • 财政年份:
    2018
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Standard Grant
Arabidopsis 2010: Analysis of the Ribonucleases of Arabidopsis Thaliana
拟南芥 2010:拟南芥核糖核酸酶分析
  • 批准号:
    1021636
  • 财政年份:
    2010
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Continuing Grant
EAGER: Development of the PARE approach for genome-wide profiling of mRNA decay in Arabidopsis
EAGER:开发用于拟南芥全基因组 mRNA 衰减分析的 PARE 方法
  • 批准号:
    0946326
  • 财政年份:
    2009
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Standard Grant
Analysis of the Ribonucleases of Arabidopsis thaliana
拟南芥核糖核酸酶分析
  • 批准号:
    0445638
  • 财政年份:
    2005
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Continuing Grant
SGER: Development of Methods for Analysis of Small Non-coding Transcrips by MPSS
SGER:通过 MPSS 分析小型非编码转录本的方法的开发
  • 批准号:
    0439186
  • 财政年份:
    2004
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Standard Grant
Analysis of the Ribonucleases of Arabidopsis thaliana
拟南芥核糖核酸酶分析
  • 批准号:
    0228144
  • 财政年份:
    2002
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Standard Grant
Analysis of the Ribonucleases of Arabidopsis thaliana
拟南芥核糖核酸酶分析
  • 批准号:
    0096394
  • 财政年份:
    2001
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Standard Grant
Conference on Plant Cell Biology being held on January 13-19, 1998; in Taos, New Mexico
植物细胞生物学会议于1998年1月13-19日举行;
  • 批准号:
    9724910
  • 财政年份:
    1998
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Standard Grant
Analysis of the Ribonucleases of Arabidopsis thaliana
拟南芥核糖核酸酶分析
  • 批准号:
    9408052
  • 财政年份:
    1994
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Continuing Grant
Acquisition of Storage-Phosphor Imaging System
获取存储荧光粉成像系统
  • 批准号:
    9016346
  • 财政年份:
    1991
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Standard Grant

相似国自然基金

基于慧眼-HXMT宽能段观测的X射线吸积脉冲星磁场研究
  • 批准号:
    12373051
  • 批准年份:
    2023
  • 资助金额:
    55.00 万元
  • 项目类别:
    面上项目
多层次纳米叠层块体复合材料的仿生设计、制备及宽温域增韧研究
  • 批准号:
    51973054
  • 批准年份:
    2019
  • 资助金额:
    60.0 万元
  • 项目类别:
    面上项目
CFHTLS-Wide和CFHTLS-Stripe82观测的弱引力透镜星系团巡天
  • 批准号:
    11103011
  • 批准年份:
    2011
  • 资助金额:
    25.0 万元
  • 项目类别:
    青年科学基金项目
精神分裂症全基因组关联研究的通路分析及验证
  • 批准号:
    81071087
  • 批准年份:
    2010
  • 资助金额:
    35.0 万元
  • 项目类别:
    面上项目
非吸烟肺癌表皮生长因子受体基因相关非编码小RNA差异表达研究
  • 批准号:
    81071914
  • 批准年份:
    2010
  • 资助金额:
    36.0 万元
  • 项目类别:
    面上项目
超高频超宽带系统射频基带补偿理论与技术的研究
  • 批准号:
    61001097
  • 批准年份:
    2010
  • 资助金额:
    22.0 万元
  • 项目类别:
    青年科学基金项目
孤独症全基因组关联第二阶段研究
  • 批准号:
    81071110
  • 批准年份:
    2010
  • 资助金额:
    32.0 万元
  • 项目类别:
    面上项目

相似海外基金

Comprehensive Harmonization and Analysis of Case/Control Whole Genome Sequencing Data from the ALS/FTD Compute Project
来自 ALS/FTD 计算项目的病例/对照全基因组测序数据的全面协调和分析
  • 批准号:
    10592917
  • 财政年份:
    2023
  • 资助金额:
    $ 56.07万
  • 项目类别:
Genome-Wide Association Analysis of salt-sensitive hypertension in elderly population
老年人盐敏感性高血压的全基因组关联分析
  • 批准号:
    495420
  • 财政年份:
    2023
  • 资助金额:
    $ 56.07万
  • 项目类别:
DATA ANALYTICS, STATISTICAL AND BIOINFORMATIC ANALYSIS AND TOOL DEVELOPMENT, Genome wide association studies (GWAS)
数据分析、统计和生物信息分析及工具开发、全基因组关联研究 (GWAS)
  • 批准号:
    10976182
  • 财政年份:
    2023
  • 资助金额:
    $ 56.07万
  • 项目类别:
MAGIC tools for genome-wide mosaic analysis with existing Drosophila resources (Equipment Supplement 2023)
利用现有果蝇资源进行全基因组嵌合分析的 MAGIC 工具(设备补充资料 2023)
  • 批准号:
    10808546
  • 财政年份:
    2023
  • 资助金额:
    $ 56.07万
  • 项目类别:
Analysis of Whole Genome Sequence and Hemostasis Phenotypes
全基因组序列和止血表型分析
  • 批准号:
    10654394
  • 财政年份:
    2023
  • 资助金额:
    $ 56.07万
  • 项目类别:
A genome-wide genealogical framework for statistical and population genetic analysis
用于统计和群体遗传分析的全基因组谱系框架
  • 批准号:
    10658562
  • 财政年份:
    2023
  • 资助金额:
    $ 56.07万
  • 项目类别:
Genome-wide Analysis of Anticoagulant Heparin Sulfate for Bioengineering Heparan
用于生物工程类乙酰肝素的抗凝剂硫酸肝素的全基因组分析
  • 批准号:
    10742641
  • 财政年份:
    2023
  • 资助金额:
    $ 56.07万
  • 项目类别:
Genome-wide analysis of the biogenesis and function of surface structures involved in interbacterial interactions
对参与细菌间相互作用的表面结构的生物发生和功能进行全基因组分析
  • 批准号:
    RGPIN-2019-06044
  • 财政年份:
    2022
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Discovery Grants Program - Individual
Genome-wide analysis of the polyamine-mediated antibiotic resistance in S. aureus
金黄色葡萄球菌多胺介导的抗生素耐药性的全基因组分析
  • 批准号:
    573803-2022
  • 财政年份:
    2022
  • 资助金额:
    $ 56.07万
  • 项目类别:
    University Undergraduate Student Research Awards
Genome wide analysis of Cryptobia salmositica
Cryptobia salmositica 的全基因组分析
  • 批准号:
    DDG-2020-00004
  • 财政年份:
    2022
  • 资助金额:
    $ 56.07万
  • 项目类别:
    Discovery Development Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了