Ecdysteroid and Retinoid Receptors in Crustacea: Expressionin Regenerating Limbs

甲壳动物中的蜕皮类固醇和类维生素A受体：在再生肢体中的表达

• 批准号: 9408342
• 负责人: Penny Hopkins
• 金额: $ 25.79万
• 依托单位: University of Oklahoma Norman Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-01 至 1998-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9408342&HistoricalAwards=false
• 关键词: Ecdysteroid; Retinoid; Receptors; Crustacea; Expressionin

9408342 Hopkins Crustaceans regenerate new walking legs when old ones are lost or injured. Ecdysteroids (the invertebrate steroid hormones) which control growth in virtually all arthropods also influence the special kind of growth involved in regeneration. The research proposed here will investigate the role of these steroids in leg regeneration in the crab, Uca pugilator. Since regeneration in crabs involves both cellular differentiation and anabolic activities, this research will yield new insights into the molecular controls of these two very different events. Using our recently isolated DNA probe for the ecdysteroid receptor, Dr. Hopkins will look at the patterns of receptor expression during regeneration and the structural/functional relationships between steroid hormones, other growth-affecting compounds (such as the retinoids), and their receptors. Because steroid receptors of invertebrates are so similar to those of higher vertebrates, the data generated by this research should provide a better understanding of how steroids, in general, control and coordinate the complicated phenomena of growth. ***

9408342霍普金斯 甲壳类动物在失去或受伤的旧腿时会再生出新的腿。 控制几乎所有节肢动物生长的蜕皮类固醇（无脊椎动物类固醇激素）也影响与再生有关的特殊生长。 这里提出的研究将调查这些类固醇在螃蟹腿再生中的作用，Uca pugilator。 由于螃蟹的再生涉及细胞分化和合成代谢活动，这项研究将对这两个非常不同的事件的分子控制产生新的见解。 利用我们最近分离的蜕皮类固醇受体的DNA探针，霍普金斯博士将研究再生过程中受体表达的模式，以及类固醇激素、其他影响生长的化合物（如类维生素A）及其受体之间的结构/功能关系。 由于无脊椎动物的类固醇受体与高等脊椎动物的类固醇受体非常相似，因此这项研究产生的数据应该能够更好地了解类固醇一般如何控制和协调复杂的生长现象。 ***