In Vitro Differentiation of Embryonal Stem Cells to Neurons

胚胎干细胞向神经元的体外分化

• 批准号: 9408787
• 负责人: David Gottlieb
• 金额: $ 3.84万
• 依托单位: Washington University School of Medicine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-08-15 至 1995-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9408787&HistoricalAwards=false
• 关键词: Vitro; Differentiation; Embryonal; Stem; Cells

A number of systems have now been developed that obviate the need for the use of animals in the scientific investigation of biological processes. This research seeks to develop a new model system for the study of neuronal differentiation in cell culture. When maintained under cell culture conditions, a small fraction of embryonal stem (ES) cells differentiate into neurons. The studies that will be performed will investigate methods to maximize the percentage of ES cells that choose the neuronal development pathway. Success in this endeavor will provide a number of significant advantages for future neurobiological research. As an example, the effects of the modification of a specific gene on neuron development can be studied in culture, without the necessity of generating animals that carry the modified gene. Further, since the developmental process under study can be continually monitored, the entire process is subject to investigation. Development of this technological advance has the potential, therefore, to be of fundamental importance in understanding the processes involved in the development of the nervous system.

现在已经开发了许多系统，这些系统消除了在生物过程的科学研究中使用动物的需求。这项研究旨在开发一种新的模型系统，以研究细胞培养中的神经元分化。 当在细胞培养条件下保持时，一小部分胚胎（ES）细胞分化为神经元。 将进行的研究将研究最大化选择神经元发育途径的ES细胞百分比的方法。 在这项工作中的成功将为未来的神经生物学研究提供许多重要的优势。 例如，可以在培养中研究特定基因对神经元发育的影响，而无需产生携带改良基因的动物。 此外，由于可以不断监控正在研究的发展过程，因此整个过程将经过研究。 因此，这种技术进步的发展具有潜力，对于理解神经系统发展的过程至关重要。