RESEARCH INITIATION AWARD: Surface Plasmon Resonance Microscope Imaging of Cell Detachment Under Hydrodynamic Shear

该项目的总体目标是应用径向流室和SPR显微镜来确定支撑基质分子粘附特性变化的影响，受体/配体相互作用，以及附着过程中细胞扩散的程度对粘附强度的影响，以及哺乳动物细胞从模型有机表面分离的机制。在分子水平上了解受体/配体介导的细胞粘附现象对于优化当前的许多生物技术是必要的，包括细胞亲和层析，用于开发用作组织培养和人工器官支持的新型生物材料，以及用于治疗涉及病理粘附行为的疾病的新的临床疗法。＊＊＊

9410215 DiMilla The overall objective of this project is to apply a radial-flow chamber and SPR microscopy to identify the effect of variations in the molecular adhesive properties of a supporting substratum, the receptor/ligand interaction, and the extent of cell spreading during attachment on the strength of adhesion and the mechanism of detachment of mammalian cells from model organic surfaces. Molecular-level insight into receptor/ligand-mediated cell adhesion phenomenon is necessary for optimizing many current biotechnologies, including cell affinity chromatography, for developing novel biomaterials for use as supports for tissue culture and artificial organs, and for implementing new clinical therapies for treating diseases involving pathological adhesive behavior. ***