RESEARCH INITIATION AWARD: Surface Plasmon Resonance Microscope Imaging of Cell Detachment Under Hydrodynamic Shear

9410215 DiMilla该项目的总体目标是应用径向流动室和SPR显微镜来识别支持基质的分子粘附特性、受体/配体相互作用和粘附期间细胞扩散程度的变化对粘附强度的影响以及哺乳动物细胞从模型有机表面分离的机制。 受体/配体介导的细胞粘附现象的分子水平的洞察力是必要的优化许多当前的生物技术，包括细胞亲和色谱，用于开发新的生物材料作为组织培养和人工器官的支持，并实施新的临床治疗涉及病理性粘附行为的疾病。 ***

9410215 DiMilla The overall objective of this project is to apply a radial-flow chamber and SPR microscopy to identify the effect of variations in the molecular adhesive properties of a supporting substratum, the receptor/ligand interaction, and the extent of cell spreading during attachment on the strength of adhesion and the mechanism of detachment of mammalian cells from model organic surfaces. Molecular-level insight into receptor/ligand-mediated cell adhesion phenomenon is necessary for optimizing many current biotechnologies, including cell affinity chromatography, for developing novel biomaterials for use as supports for tissue culture and artificial organs, and for implementing new clinical therapies for treating diseases involving pathological adhesive behavior. ***