New Strategies for the Production of Efficient Catalytic Antibodies

这个项目正在研究开发生产催化抗体的新策略。这些策略是基于大肠杆菌细胞表面大量不同抗体的表达，然后通过筛选程序从人群中选择所需的分子。选择过程将利用过渡态类似物的荧光标记偶联物与细胞表面抗体之间可能的结合。结合到过渡态模拟物的细胞可以通过荧光激活细胞分选（FACS）来选择。在一项平行研究中，本研究将试图证明直接基于产物形成速率而不是结合过渡态类似物来分离催化抗体。催化抗体具有高度的选择性和活性，但由于分离和生产成本高，目前用于商业目的的成本过高。该项目的一部分内容是寻找降低成本的新方法。＊＊＊

9412502 Georgiou This project is on research to develop new strategies for the production of catalytic antibodies. The strategies are based on the expression of a vast number of different antibodies on the E. coli cell surface followed by screening procedures for selecting the desired molecules from the population. The selection process will make use of the possible binding between the fluorescently labeled conjugate of the transition state analog and the antibody on the cell surface. Cells bound to the transition state analog can then be selected by fluorescent activated cell sorting (FACS). In a parallel study, this research will attempt to demonstrate the isolation of catalytic antibodies directly based on the rate of product formation rather than binding to transition state analogs. Catalytic antibodies have a high degree of selectivity and activity, but are currently prohibitively expensive for commercial purposes because of the high cost of isolation and production. Part of this project is on new approaches to lower this cost. ***