Engineering of Bacterial Surfaces

该项目的目标是进一步开发一种基于细菌表面定向修饰的新技术。细胞表面的性质在很大程度上取决于外部暴露的受体和酶的存在。到目前为止，还不可能在细菌表面定位蛋白质。最近，首席研究员（pi）已经证明，酶活性重组蛋白可以锚定在大肠杆菌和其他革兰氏阴性菌的表面。这为许多生物技术应用开辟了道路，包括：(i)生产由重组受体包衣的细胞组成的廉价细胞吸附剂；生产新的微生物生物催化剂，由表达表面固定酶的细胞组成，以代替固定化酶颗粒；（三）开发哺乳动物免疫系统的细菌类似物，用于选择具有所需特性的抗体。

The objective of this project is to further develop a new technology based on the directed modification of bacterial surfaces. The properties of cellular surfaces are determined to a large extent by the presence of externally exposed receptors and enzymes. Until now, it has not been possible to localize proteins on the bacterial surface. Recently, the Principal Investigators (PIs) have demonstrated that enzymatically active recombinant proteins can be anchored on the surface of E. coli and other gram negative bacteria. This opens the way for numerous biotechnology applications including: (i) Generation of inexpensive cellular adsorbents consisting of cells coated with recombinant receptors; (ii) Production of new microbial biocatalysts consisting of cells expressing surface anchored enzymes for use in lieu of immobilized enzyme particles; and (iii) Development of bacterial analogues of the mammalian immune system suitable for the selection of antibodies with desired properties.