U.S.-Poland Cooperative Science: Design and Chemistry of Modified Nucleosides for Nucleic Acid Synthesis

这个由北卡罗来纳州立大学的Paul F. Agris博士和罗兹工业大学的Andrzej Malkiewicz博士合作的美国-波兰研究项目是关于“用于核酸合成的修饰核苷的设计和化学”。修饰核苷的化学、结构和动力学如何与rna和DNA的功能相关，目前还不清楚。一旦理解，即使是少量的碱基修饰核苷，这种不同的化学和结构的集合也可以应用于对生物学和医学重要的分子的设计和工程。rna和DNA中80多种修饰核苷的进化与它们的位点选择定位和核酸化学和结构的改变有关；实现这一目标的可能性与改性的化学和结构一样多种多样。合作研究人员将开发新的技术和方法，研究天然和非天然修饰和稳定同位素标记的核苷在RNA和DNA中的定点结合，初步适用于基础核酸生物化学（分子进化，核酸结构）。合作项目的互利源于双方研究人员经验的完美互补。Malkiewicz博士在有机化学合成、化学和分析修饰核苷和含有修饰核苷的小低聚物方面经验丰富；而Agris博士在RNA中修饰核苷的结构和功能的生物合成和分析方面有着广泛的经验。该生物化学项目通过使波兰和美国的领先专家在共同感兴趣和能力强的领域结合互补人才和汇集资源，实现了推进科学知识的计划目标。＊＊＊

9412482 Agris This US-Poland Research project between Dr. Paul F. Agris of North Carolina State University, and Dr. Andrzej Malkiewicz of the Technical University of Lodz, is on "Design and Chemistry of Modified Nucleosides for Nucleic Acid Synthesis." How modified nucleoside chemistry, structure and dynamics relate to function of RNAs, but also DNA, is not understood. Once understood, even for a small number of base modified nucleosides, this varied collection of chemistries and structures can be applied to the design and engineering of molecules important to biology and medicine. The evolution of the more than 80 modified nucleosides in RNAs and DNA is connected with their site-selected positioning and altering of nucleic acid chemistry and structure; the potential to accomplish this is as varied as are the chemistries and structures of the modifications. The cooperating investigators will develop new technologies and methods for studying the site-selected incorporation of both native and non- native modified and stable-isotope labeled nucleosides in RNA and DNA, applicable, initially, to fundamental nucleic acid biochemistry (molecular evolution, nucleic acid structure). The mutual benefit from the cooperative project derives from the perfect complementary experiences of the two investigators. Dr. Malkiewicz is broadly experienced in the organic chemical syntheses, chemistry and analyses of modified nucleosides and small oligomers containing modified nucleosides; whereas Dr. Agris is broadly experienced in the biosynthesis and analyses of both the structures and functions of modified nucleosides in RNA. This project in biochemistry fulfills the program objective of advancing scientific knowledge by enabling leading experts in Poland and the United States to combine complementary talents and pool resources in areas of strong mutual interest and competence. ***