NASA/NSF Collaborative Research: Evaluating Phytohormone Signal Transduction Using Xenopus oocytes

NASA/NSF 合作研究：利用非洲爪蟾卵母细胞评估植物激素信号转导

• 批准号: 9416035
• 负责人: Fedora Sutton
• 金额: $ 63.21万
• 依托单位: South Dakota State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-09-15 至 2000-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9416035&HistoricalAwards=false
• 关键词: NASA; NSF; Collaborative; Research; Evaluating

9416035 Sutton The long-term objective of the proposed research is to identify signal transduction components involved in plant response to environmental stimuli. Using the Xenopus oocyte/mRNA injection system, research proposed will focus on signal transduction in response to the environmental stimuli of low non-freezing temperature and dehydration in higher plants. A common effect of these two stimuli on plants is to modulate endogenous abscisic acid (ABA) levels, not all responses to low temperature are ABA dependent. The mode of action of ABA has not been conclusively demonstrated. Questions remain regarding actual location of ABA receptors, and second messengers, if any, that are necessary for signal transduction. Research proposed will initially focus on ABA, since there is evidence in the literature of ABA stimulating Ca release. Preliminary data from this lab suggests that ABA may interact with plasma membrane receptors and cause an increase in intracellular Ca. Specific aims of this proposal are: 1) To characterize the responses, to ABA and several ABA analogues, from oocytes injected with tobacco mRNA. 2) If Ca is mobilized, to localize its source in an attempt to determine the manner in which ABA and its receptors are functioning. If Ca is not mobilized, to determine the alternative second messenger system being utilized by ABA. 3) To utilize the Xenopus oocyte system to isolate and characterize ABA receptors cDNA clones. Knowing how ABA and receptor(s) interact will help in understanding plant responses common to the stimuli of low temperature and dehydration. Research proposed will involve the areas of electrophysiology, molecular biology, biochemistry and cell biology. The integrative nature of this research will make it possible for research to be performed at appropriate collaborative research laboratories and will prove to be a rewarding and exciting environment for training of postdoctoral associates and graduate students. ** *

9416035萨顿提出的研究的长期目标是确定参与植物对环境刺激的反应的信号转导成分。 利用非洲爪蟾卵母细胞/mRNA注射系统，研究高等植物对低温和脱水环境刺激的信号转导。 这两种刺激对植物的共同作用是调节内源脱落酸（阿坝）水平，并非所有对低温的反应都依赖于阿坝。 阿坝的作用方式尚未得到最终证实。 关于阿坝受体和第二信使（如果有的话）的实际位置仍然存在问题，这是信号转导所必需的。 建议的研究将首先集中在阿坝，因为有证据表明阿坝刺激钙释放的文献。 该实验室的初步数据表明，阿坝可能与质膜受体相互作用，导致细胞内Ca增加。 本研究的具体目的是：1）研究烟草mRNA对卵母细胞阿坝和几种阿坝类似物的反应。2)如果钙被动员，则定位其来源，试图确定阿坝及其受体的功能方式。 如果Ca未被动员，则确定阿坝所利用的替代第二信使系统。 3)利用非洲爪蟾卵母细胞系统分离和鉴定阿坝受体cDNA克隆。 了解阿坝和受体如何相互作用将有助于理解植物对低温和脱水刺激的共同反应。 拟进行的研究将涉及电生理学、分子生物学、生物化学和细胞生物学等领域。 这项研究的综合性将使研究有可能在适当的合作研究实验室进行，并将被证明是一个有益的和令人兴奋的环境，为博士后助理和研究生的培训。 ** *