EAGER: Control of Animal and Plant ABA receptor interaction with Associated G-Protein

名称：Fedora sutton提案#:IOS 0940858标题：EAGER：控制动植物ABA受体与相关g蛋白脱落酸（ABA）的相互作用是植物自然产生的一种激素，用于调节胁迫反应，包括干旱时期的气孔关闭。ABA受体的鉴定已经进行了20多年。到目前为止，还没有在动物中发现ABA受体；然而，胰腺细胞已被证明产生ABA并对ABA作出反应，这表明存在胰腺ABA受体。研究人员提出胰腺ABA受体与其他蛋白质相互作用以发挥功能。因此，研究人员提出利用酵母双杂交分子技术，利用相互作用蛋白分离ABA受体。如果这个假设是正确的，研究人员将确定一种动物的ABA受体和相应的调节因子。以下几个方面是该项目的更广泛影响的工作。该项目活动包括两名本科生和两名女科学家的教育。最近的研究表明，ABA可能在动物细胞中发挥重要的调节作用，包括通过ABA信号通路在人和大鼠胰腺细胞中激活人粒细胞和刺激胰岛素释放。因此，对这些信号通路的进一步了解不仅可以为植物带来额外的耐旱策略，还可以随之而来的是人类II型糖尿病和肥胖症的治疗。因此，该项目在环境压力下可能影响农业植物生产力的领域以及在人类健康领域具有更广泛的影响。

Name: Fedora SuttonProposal #: IOS 0940858Title: EAGER: Control of Animal and Plant ABA receptor interaction withAssociated G-Protein Abscisic acid (ABA) is a hormone naturally produced in plants to regulate stress responses, including stomatal closure in times of drought. Identification of the ABA receptor has been pursued for more than 20 years. To date, no ABA receptors have been identified in animals; however, pancreatic cells have been shown to produce ABA and respond to ABA suggesting the presence of a pancreatic ABA receptor. The investigators propose that the pancreatic ABA receptor interacts with other proteins in order to function. As a result the investigators propose using the interacting proteins to isolate the ABA receptor by use of the yeast two-hybrid molecular technique. If the hypothesis is correct, the researchers will identify an animal ABA receptor and corresponding regulators. The following aspects of the project are broader impacts of the work. The project activity includes the education of two undergraduates and two women scientists. Recent findings suggest that ABA may play an important regulatory role in animal cells, including activation of human granulocytes and stimulation of insulin release through an ABA signaling pathway in human and rat pancreatic cells. Therefore, not only could the increased understanding of these signaling pathways lead to additional drought-tolerance strategies in plants, treatments for Type II diabetes and obesity in humans could also follow. Thus, the project has broader impacts in the area of potentially impacting agricultural plant productivity under environmental stress, and also in the area of human health.