EAGER: Control of Animal and Plant ABA receptor interaction with Associated G-Protein

姓名（N）：Fedora Sutton提案编号：脱落酸（阿坝）是植物中天然产生的一种激素，用于调节逆境反应，包括干旱时气孔的关闭。阿坝受体的鉴定已经进行了20多年。到目前为止，没有阿坝受体已被确定在动物中，然而，胰腺细胞已被证明产生阿坝和响应阿坝表明胰腺阿坝受体的存在。研究人员提出，胰腺阿坝受体与其他蛋白质相互作用以发挥功能。因此，研究人员建议使用相互作用的蛋白质分离阿坝受体的酵母双杂交分子技术。如果假设是正确的，研究人员将确定一种动物阿坝受体和相应的调节剂。该项目的以下方面是工作的更广泛影响。项目活动包括培养两名大学生和两名女科学家。最近的研究结果表明，阿坝可能在动物细胞中发挥重要的调节作用，包括激活人粒细胞和刺激胰岛素释放通过阿坝信号通路在人类和大鼠胰腺细胞。因此，不仅可以增加对这些信号通路的理解，从而在植物中产生额外的耐旱策略，还可以随后治疗人类的II型糖尿病和肥胖症。因此，该项目在环境压力下可能影响农业植物生产力的领域以及在人类健康领域具有更广泛的影响。

Name: Fedora SuttonProposal #: IOS 0940858Title: EAGER: Control of Animal and Plant ABA receptor interaction withAssociated G-Protein Abscisic acid (ABA) is a hormone naturally produced in plants to regulate stress responses, including stomatal closure in times of drought. Identification of the ABA receptor has been pursued for more than 20 years. To date, no ABA receptors have been identified in animals; however, pancreatic cells have been shown to produce ABA and respond to ABA suggesting the presence of a pancreatic ABA receptor. The investigators propose that the pancreatic ABA receptor interacts with other proteins in order to function. As a result the investigators propose using the interacting proteins to isolate the ABA receptor by use of the yeast two-hybrid molecular technique. If the hypothesis is correct, the researchers will identify an animal ABA receptor and corresponding regulators. The following aspects of the project are broader impacts of the work. The project activity includes the education of two undergraduates and two women scientists. Recent findings suggest that ABA may play an important regulatory role in animal cells, including activation of human granulocytes and stimulation of insulin release through an ABA signaling pathway in human and rat pancreatic cells. Therefore, not only could the increased understanding of these signaling pathways lead to additional drought-tolerance strategies in plants, treatments for Type II diabetes and obesity in humans could also follow. Thus, the project has broader impacts in the area of potentially impacting agricultural plant productivity under environmental stress, and also in the area of human health.