Serrate: Neurogenic interactions and intercellular signaling

小行星9418678 黑腹果蝇中的一种突变表现出神经元增生，伴随着皮下结构的减少，这种突变被命名为Notch。另一种基因突变Serrate似乎在与Notch基因产物相互作用中发挥重要的发育作用，以在早期胚胎成神经细胞以外的组织发育期间介导细胞间信号传导，其中另一种基因Delta的产物似乎与Notch相互作用。由于丝氨酸无效等位基因导致幼虫死亡，它一直无法确定哪些组织需要丝氨酸的活力或可能的作用，丝氨酸胚胎后。新的方法将被用来回答问题：（1）。Serrate在开发过程中发挥的重要作用是什么？ （2.）Serrate蛋白在发育过程中何时何地表达？（3.）与无效Delta等位基因强烈相互作用的显性锯齿突变的性质是什么？ 本实验旨在研究果蝇（Drosophila melanogaster）Serrate基因产物的功能。许多遗传基因座已被分离的基础上，一个共同的突变体表型和研究表明，这些基因的产品中的大多数是相互作用的方式所必需的分化的神经外胚层。其中之一，锯齿，将检查其在胚胎发育中的功能，并在与其他相关基因位点的产品相互作用的方式。 ***

9418678 Fleming One mutation in Drosophila melanogaster which exhibits neuronal hyperplasia with concomitant reduction in hypodermal structures has been named Notch. Serrate, another gene mutation, appears to play an important developmental role in interacting with the Notch gene product to mediate cell- to- cell signaling during the development of tissues other than the early embryonic neuroblast, where the product of another gene, Delta, seems to interact with Notch. Since Ser-null alleles cause larval death, it has not been possible to identify which tissues require Ser for viability or the possible role of Ser post-embryonically. New approaches will be utilized to answer the questions: (1.) What is the essential role played by Serrate during development? (2.) Where and when is the Serrate protein expressed during development? (3.) What is the nature of the class of dominant Serrate mutations that interact strongly with null Delta alleles? %%% Experiments are proposed to investigate the function of the Serrate gene product in the fruit fly, Drosophila melanogaster. A number of genetic loci have been isolated on the basis of a common mutant phenotype and studies have suggested that most of these genes products are interactive in a manner necessary for the differentiation of neuro-ectoderm. The function of one of them, Serrate, will be examined in relation to its function in embryonic development and in the manner in which it interacts with products of other related gene loci. ***