Serrate: Neurogenic interactions and intercellular signaling

锯齿：神经源性相互作用和细胞间信号传导

• 批准号: 9418678
• 负责人: Robert Fleming
• 金额: $ 33.63万
• 依托单位: University of Rochester
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-01-01 至 1998-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9418678&HistoricalAwards=false
• 关键词: Serrate; Neurogenic; interactions; intercellular; signaling

9418678 Fleming One mutation in Drosophila melanogaster which exhibits neuronal hyperplasia with concomitant reduction in hypodermal structures has been named Notch. Serrate, another gene mutation, appears to play an important developmental role in interacting with the Notch gene product to mediate cell- to- cell signaling during the development of tissues other than the early embryonic neuroblast, where the product of another gene, Delta, seems to interact with Notch. Since Ser-null alleles cause larval death, it has not been possible to identify which tissues require Ser for viability or the possible role of Ser post-embryonically. New approaches will be utilized to answer the questions: (1.) What is the essential role played by Serrate during development? (2.) Where and when is the Serrate protein expressed during development? (3.) What is the nature of the class of dominant Serrate mutations that interact strongly with null Delta alleles? %%% Experiments are proposed to investigate the function of the Serrate gene product in the fruit fly, Drosophila melanogaster. A number of genetic loci have been isolated on the basis of a common mutant phenotype and studies have suggested that most of these genes products are interactive in a manner necessary for the differentiation of neuro-ectoderm. The function of one of them, Serrate, will be examined in relation to its function in embryonic development and in the manner in which it interacts with products of other related gene loci. ***

在黑腹果蝇中，一种表现出神经元增生并伴随皮下结构减少的突变被命名为Notch。Serrate，另一个基因突变，似乎在与Notch基因产物相互作用中发挥重要的发育作用，在组织发育过程中介导细胞间信号传导，而不是早期胚胎神经母细胞，其中另一个基因的产物，Delta，似乎与Notch相互作用。由于Ser-null等位基因会导致幼虫死亡，因此不可能确定哪些组织需要Ser维持活力或Ser在胚胎后可能发挥的作用。将采用新的方法来回答以下问题：(1)Serrate在开发过程中扮演了什么重要角色？(2)。在发育过程中，锯齿蛋白何时何地表达？(3)。与零δ等位基因强烈相互作用的显性锯齿突变的性质是什么？提出了在果蝇（Drosophila melanogaster）中研究Serrate基因产物功能的实验。在共同突变表型的基础上，已经分离出许多基因位点，研究表明，这些基因产物中的大多数在神经外胚层分化所需的方式上相互作用。其中的一个，Serrate的功能，将被检查其在胚胎发育中的功能，以及它与其他相关基因位点的产物相互作用的方式。＊＊＊