Serrate: Neurogenic interactions and intercellular signaling

9418678黑腹果蝇的一个突变体命名为Notch，它表现出神经元的增殖并伴随着皮下结构的减少。锯齿状是另一种基因突变，在除早期胚胎神经母细胞外的其他组织的发育过程中，它似乎在与Notch基因产物相互作用的过程中发挥着重要的发育作用。在早期胚胎神经母细胞中，另一基因Delta的产物似乎与Notch相互作用。由于丝氨酸缺失等位基因导致幼虫死亡，目前还不可能确定哪些组织需要丝氨酸才能存活，或者丝氨酸在胚胎后可能发挥的作用。将采用新的方法回答以下问题：(1)撒拉特在发展过程中扮演的重要角色是什么？在发育过程中，锯齿状蛋白在何时何地表达？这类与零Delta等位基因相互作用强烈的显性锯齿状突变的性质是什么？我们提出了%的实验来研究果蝇中锯齿状基因产物的功能。根据一种常见的突变表型，已分离出许多遗传位点，研究表明，这些基因产物中的大多数都是相互作用的，对于神经外胚层的分化是必要的。其中一个基因Serrate的功能将被检测与其在胚胎发育中的功能以及它与其他相关基因座产物相互作用的方式有关。***

9418678 Fleming One mutation in Drosophila melanogaster which exhibits neuronal hyperplasia with concomitant reduction in hypodermal structures has been named Notch. Serrate, another gene mutation, appears to play an important developmental role in interacting with the Notch gene product to mediate cell- to- cell signaling during the development of tissues other than the early embryonic neuroblast, where the product of another gene, Delta, seems to interact with Notch. Since Ser-null alleles cause larval death, it has not been possible to identify which tissues require Ser for viability or the possible role of Ser post-embryonically. New approaches will be utilized to answer the questions: (1.) What is the essential role played by Serrate during development? (2.) Where and when is the Serrate protein expressed during development? (3.) What is the nature of the class of dominant Serrate mutations that interact strongly with null Delta alleles? %%% Experiments are proposed to investigate the function of the Serrate gene product in the fruit fly, Drosophila melanogaster. A number of genetic loci have been isolated on the basis of a common mutant phenotype and studies have suggested that most of these genes products are interactive in a manner necessary for the differentiation of neuro-ectoderm. The function of one of them, Serrate, will be examined in relation to its function in embryonic development and in the manner in which it interacts with products of other related gene loci. ***