Acquisition of HPLC System

购置高效液相色谱系统

• 批准号: 9419614
• 负责人: Brenda Speer
• 金额: $ 2.4万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9419614&HistoricalAwards=false
• 关键词: Acquisition; HPLC; System

This is a shared equipment proposal for the acquisition of a high performance liquid chromatography system (HPLC). HPLC provides a major improvement over conventional chromatography by increasing resolution, improving yields, and decreasing elution times and volumes. HPLC separations are applicable to virtually any class of compound, including small organic molecules, lipids, nucleic acids or macromolecules such as proteins, due to the diversity of available columns. The requested HPLC consists of the basic components required to provide a fully functional and highly versatile semi-preparative system. The unit includes an IBM 486 computer with the appropriate software for control of the individual HPLC components and data analysis; a single two solvent pump system capable of gradient formation; a manual sample injector; a Diode array detector for monitoring elution of a wide variety of molecules the Diode array permits the user to scan multiple wavelengths during an experiment, which will allow the researcher to determine if the eluted fraction is pure or not ; the Quick Res software for analysis of co-eluting compounds; and an initial assortment of HPLC columns to allow separations based on different physical properties. The requested instrument will enhance and modernize facilities available for research and teaching in the department of Biochemistry, Microbiology and Molecular Biology in addition to being available to other faculty and students outside of the department. The HPLC system will directly benefit several faculty research projects such as: 1) the metabolism and degradation of tetracycline and hydrocarbons by bacteria; 2) mechanisms regulating mammalian proteolytic enzymes, in vivo and in vitro; 4) post-translational modification as a regulatory mechanism in signal transduction processes in Dictyostelium; 5) purification of phenolics and hormones; 6) studies of the intermediate and slow blocks against polyspermy and embryonic stress tolerance in f ucoid algae and 7) the regulation of membrane lipid and fatty acid composition and the metabolism of these molecules in a variety of eubacteria, eukaryotes and plants. The graduate and undergraduate curricula in Biochemistry, Microbiology and Molecular Biology at the University of Maine include several laboratory courses that will also be enhanced by the availability of a HPLC system. Proposed uses include purification of monoclonal antibodies for Immunology; separation and isolation of DNA and polymerase chain reaction products for Molecular Biology and Bacterial Genetics; purification of proteins and peptide mapping in Biochemistry; and characterization of membrane lipids from several microorganisms for Bacterial Physiology. The inclusion of HPLC within these courses will provide our students with an important experience in using current methodologies. Such skills are commonly required and are greatly beneficial for ensuring their competitiveness for post-graduate studies, research jobs or careers.

这是一项收购高效液相色谱系统(HPLC)的共享设备提案。通过提高分离度、提高产率、减少洗脱时间和体积，高效液相色谱法提供了比传统层析更大的改进。由于可用色谱柱的多样性，高效液相色谱分离几乎适用于任何一类化合物，包括小有机分子、脂类、核酸或蛋白质等大分子。所要求的高效液相色谱包括提供全功能和高度通用的半制备系统所需的基本组件。该装置包括一台IBM 486计算机，其上安装了适当的软件来控制单个高效液相色谱成分并进行数据分析；一个单独的双溶剂泵系统能够进行梯度形成；一个手动进样器；一个二极管阵列检测器，用于监测各种分子的洗脱；二极管阵列允许用户在实验期间扫描多个波长，这将使研究人员能够确定洗脱部分是否纯净；Quick RES软件用于共洗脱化合物的分析；以及初始分类的高效液相色谱柱，以便根据不同的物理性质进行分离。所要求的仪器将加强和现代化生物化学、微生物学和分子生物学系现有的研究和教学设施，并向系外的其他教职员工和学生提供。高效液相系统将直接惠及几个教职员工的研究项目，如：1)细菌对四环素和碳氢化合物的代谢和降解；2)在体内和体外调节哺乳动物蛋白水解酶的机制；4)翻译后修饰作为竹鞭毛藻信号转导过程中的调控机制；5)酚类和激素的纯化；6)丝状藻类中抗多精受精和胚胎逆境耐受的中速和慢速阻挡的研究；7)各种真细菌、真核生物和植物中膜脂和脂肪酸组成的调节以及这些分子的新陈代谢。缅因州大学生物化学、微生物学和分子生物学的研究生和本科生课程包括几门实验室课程，这些课程也将因高效液相系统的提供而得到加强。建议的用途包括纯化免疫学的单抗；分离和分离DNA和聚合酶链式反应产物，用于分子生物学和细菌遗传学；纯化蛋白质和生物化学中的肽图；以及表征几种微生物的膜脂，用于细菌生理学。在这些课程中加入高效液相色谱将为我们的学生提供使用当前方法的重要经验。这些技能是普遍需要的，对确保他们在研究生学习、研究工作或职业生涯中的竞争力大有裨益。