Construction and Evaluation of a Step Scanning Interferometric Instrument for the Observation of Infrared Circular Dichroism

红外圆二色性观测步进扫描干涉仪的构建与评价

• 批准号: 9420053
• 负责人: Max Diem
• 金额: $ 13.64万
• 依托单位: CUNY Hunter College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-03-01 至 1998-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9420053&HistoricalAwards=false
• 关键词: Construction; Evaluation; Step; Scanning; Interferometric

The following proposal is aimed at developing a prototype instrument for the observation of infrared, or vibrational Circular Dichroism (VCD), using Step-Scanning Fourier transform (SS-FT) methodology. VCD is a spectroscopic technique, developed originally in the mid 1970's, in which the differential absorption of left and right circularly polarized indrared light by vibrational transitions is observed. This effect is very small, yet contains conformational and structural information which cannot be obtained easily by any other spectroscopic method. The lack of commercial instrumentation for its observation has restricted the wider application of VCD as a structural tool. This proposal is intended to produce a new generation of VCD instruments which could serve as blueprints for commercial VCD spectrometers. The proposed instrument is conceived as a VCD spectrometer, rather than a Fourier Transform infrared spectrometer to which VCD capabilities were added. A number of technical innovations will make this instrument very unique, and will extend its capabilities beyond the present limits of VCD spectroscopy. The applicant's laboratory has been in the forefront of the development of vibrational optical activity, and has extensive experience with both dispersive and interferomatic spectroscopic methods. Thus, this laboratory is ideally suited to take a leading role in development of a new generation of VCD spectrometers. The main thrust of the applicant's research is the determination of the solution conformation of biological model systems: peptides and small proteins, as well asoligonucleotides and their interactions with drugs. The SS- FT-VCD instrument described here will be used in these studies to further reduce VCD data acquisition time, improve spectral quality and permit spectra to be collected from more dilute aqueous solutions.

以下建议旨在开发一种原型仪器，用于使用步进扫描傅里叶变换（SS-FT）方法观察红外或振动圆二色性（VCD）。VCD是一种光谱技术，最初发展于20世纪70年代中期，其中通过振动跃迁观察到左和右圆偏振入射光的差分吸收。 这种影响是非常小的，但包含构象和结构的信息，不能很容易地获得任何其他光谱方法。 由于缺乏商业化的观测仪器，限制了VCD作为构造工具的广泛应用。 该提案旨在生产新一代VCD仪器，可作为商用VCD光谱仪的蓝图。 拟议的仪器被认为是一个VCD光谱仪，而不是一个傅立叶变换红外光谱仪，其中VCD的能力被添加。 许多技术创新将使这台仪器非常独特，并将其能力扩展到目前VCD光谱的极限之外。 申请人的实验室一直处于振动光学活性开发的前沿，并且在色散和干涉光谱方法方面具有丰富的经验。 因此，该实验室非常适合在新一代VCD光谱仪的开发中发挥主导作用。 申请人研究的主要重点是确定生物模型系统的溶液构象：肽和小蛋白质，以及寡核苷酸及其与药物的相互作用。 本文所述的SS-FT-VCD仪器将用于这些研究，以进一步减少VCD数据采集时间，提高光谱质量，并允许从更稀的水溶液中收集光谱。