Virus Expression in Plants

9421002豪厄尔 该项目的目标是确定负责花椰菜花叶病毒（CaMV）运动中宿主作用的基因，并在此过程中找到赋予病毒抗性的植物基因。提出了两种方法。1)将分离和表征拟南芥的病毒抗性突变体，2）将选择对缺陷植物病毒敏感的拟南芥抑制突变体。第二种方法是新颖的，因为它允许对易感植物进行正选择，这些植物抑制任何选定的病毒基因或给定基因的任何特定功能中的突变。如果成功，它将为植物抗病基因的选择开辟新的途径。 该提案描述了一种新的方法来研究植物-病毒相互作用和鉴定参与病毒复制和运动的宿主功能。选择易受缺陷型CaMV感染的拟南芥属植物。由于宿主和病毒之间的相互作用在病毒运动中最为明显，因此将产生使CaMV运动蛋白失能的突变。 此外，将选择病毒抗性突变体，并将表征病毒抗性性状，显然是一种单基因性状，其已被证明可阻断病毒的长距离移动。 ***

9421002 Howell The goal of this project is to identify genes responsible for the role of the host in the movement of cauliflower mosaic virus (CaMV) and in the process to find plant genes that confer virus resistance. Two approaches are proposed. 1)Virus resistance mutants of Arabidopsis will be isolated and characterized and 2)Arabidopsis suppresser mutants susceptible to defective plant viruses will be selected. The second approach is novel in that it allows positive selection for susceptible plants that suppress mutations in any chosen viral gene or in any particular function of a given gene. If successful, it will open new avenues for the selection of genes important in plant disease resistance. %%% This proposal describes a novel approach to the study of plant-virus interactions and the identification of host functions involved in virus replication and movement in plants. Arabidopsis plants that are susceptible to infection by defective CaMV will be selected. Because the interplay between host and virus is most apparent in virus movement, mutations that disable the CaMV movement protein will be created. In addition, virus resistant mutants will be selected and a virus-resistant trait, apparently a one-gene trait, which has been shown to block long-distance virus movement will be characterized. ***