Genetic and Molecular Analysis of Neurotransmitter Release in C. elegans

线虫神经递质释放的遗传和分子分析

• 批准号: 9421505
• 负责人: Aixa Alfonso
• 金额: $ 29.66万
• 依托单位: University of Iowa
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-15 至 1996-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9421505&HistoricalAwards=false
• 关键词: Genetic; Molecular; Analysis; Neurotransmitter; Release

9421505 Alfonso Communication between cells of the nervous system (called neurons) is done via chemicals called neurotransmitters. This chemical communication (synaptic transmission) involves packaging of the neurotransmitter into vesicles that are released when the neuron is activated. The goal of this project is to understand the molecular and genetic bases of release of these vesicles. Synaptic transmission is being studied in the soil nematode Caenorhabditis elegans, where the investigator has identified new proteins involved in the neurotransmitter vesicular release through the isolation of mutations in the genes that encode these proteins. Animals with mutations in these gene have defects in synaptic transmission mediated by the chemical neurotransmitters acetylcholine and gamma-aminobutyric acid. These studies will give important insights into the regulation of vesicular release from neurons and also vesicle-mediated exocytosis in other cell types.

9421505阿方索 神经系统细胞（称为神经元）之间的通信是通过称为神经递质的化学物质完成的。 这种化学通讯（突触传递）涉及神经递质包装成囊泡，当神经元被激活时释放。 该项目的目标是了解这些囊泡释放的分子和遗传基础。 突触传递正在土壤线虫秀丽隐杆线虫中进行研究，研究人员通过分离编码这些蛋白质的基因突变，确定了参与神经递质囊泡释放的新蛋白质。 这些基因突变的动物在由化学神经递质乙酰胆碱和γ-氨基丁酸介导的突触传递中存在缺陷。 这些研究将提供重要的见解泡释放神经元和泡介导的胞吐在其他类型的细胞的调节。