Electrophoresis and DNA Manipulation Equipment

这个项目的目的是让我们的学生体验最先进的仪器，并增加他们对分子生物学和基因操作的熟悉程度。为了解决这些问题，我们建议为我们的普通生物实验室配备六个电泳站，用于操作DNA限制性片段，一个微离心机和用于DNA分离和转化实验的培养箱。这个设备将允许我们的学生进行分子生物学中常见的所有基本类型的操作。学生将在一系列的实验室中研究和执行DNA分离和细菌转化和DNA限制性内切酶消化的重组DNA技术。这些实验室将为我们的学生提供实验仪器操作和研究性实验的直接经验。这些实验将在我们的生物学专业课程、非专业的普通生物学课程和微生物学课程中进行。

9451889 Trimble The objectives of this project are to give our students experience with state of the art instrumentation and to increase their familiarity with molecular biology and genetic manipulation. To address these issues, we propose to equip our general biology laboratory with six electrophoresis stations for the manipulation of DNA restriction fragments, a microcentrifuge and incubator for DNA isolation and for transformation experiments. This equipment would allow our students to conduct all of the basic types of manipulations common in molecular biology. Students, in a series of laboratories, will investigate and perform DNA isolations and the recombinant DNA techniques of bacterial transformation and DNA restriction enzyme digests. These laboratories will provide our students with direct experience in the operation of experimental instrumentation and in the performance of investigative experiments. The laboratories will be performed in our biology major courses, our non-major general biology courses and in microbiology.