Electrophoresis and DNA Manipulation Equipment

电泳和 DNA 操作设备

• 批准号: 9451889
• 负责人: Dorothea Trimble
• 金额: $ 0.43万
• 依托单位: College of the Sequoias
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1996-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9451889&HistoricalAwards=false
• 关键词: Electrophoresis; DNA; Manipulation; Equipment

9451889 Trimble The objectives of this project are to give our students experience with state of the art instrumentation and to increase their familiarity with molecular biology and genetic manipulation. To address these issues, we propose to equip our general biology laboratory with six electrophoresis stations for the manipulation of DNA restriction fragments, a microcentrifuge and incubator for DNA isolation and for transformation experiments. This equipment would allow our students to conduct all of the basic types of manipulations common in molecular biology. Students, in a series of laboratories, will investigate and perform DNA isolations and the recombinant DNA techniques of bacterial transformation and DNA restriction enzyme digests. These laboratories will provide our students with direct experience in the operation of experimental instrumentation and in the performance of investigative experiments. The laboratories will be performed in our biology major courses, our non-major general biology courses and in microbiology.

9451889 Trimble 该项目的目标是让我们的学生体验最先进的仪器，并提高他们对分子生物学和基因操作的熟悉程度。为了解决这些问题，我们建议为我们的普通生物学实验室配备六个用于操作 DNA 限制性片段的电泳站、一个微量离心机和用于 DNA 分离和转化实验的培养箱。该设备将使我们的学生能够进行分子生物学中常见的所有基本类型的操作。学生将在一系列实验室中研究和实施 DNA 分离以及细菌转化和 DNA 限制性酶消化的重组 DNA 技术。这些实验室将为我们的学生提供操作实验仪器和进行研究实验的直接经验。实验室将在我们的生物学专业课程、非专业普通生物学课程和微生物学中进行。