Single-molecule visualization and manipulation of protein and DNA interactions and dynamics, on nanoscale dimensions

纳米尺度上蛋白质和 DNA 相互作用和动力学的单分子可视化和操纵

• 批准号: RGPIN-2017-04348
• 负责人: Leslie, Sabrina
• 金额: $ 3.35万
• 依托单位: McGill University
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2017
• 资助国家: 加拿大
• 起止时间: 2017-01-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=633265
• 关键词: Single; molecule; visualization; manipulation; protein

The overarching objective of my research program is to visualize, quantitatively analyze and understand protein-DNA and protein-protein interactions, which play key biophysical roles in regulating cellular processes. I am fascinated by the interplay between the dynamic, structural, and genetic properties of biopolymers such as protein and DNA molecules, which advancements in visualization and nano manipulation tools, as well as theoretical treatments of confined molecules, are allowing us to understand in further detail. This Discovery Grant will enable my group to leverage our single-molecule microscopy tools to explore open questions about the behavior of protein and DNA systems on nanoscale dimensions; questions which are inaccessible to other methods, and which have international research interest and impact.

我的研究项目的首要目标是可视化、定量分析和理解蛋白质-DNA 和蛋白质-蛋白质相互作用，它们在调节细胞过程中发挥着关键的生物物理作用。我对蛋白质和 DNA 分子等生物聚合物的动态、结构和遗传特性之间的相互作用着迷，可视化和纳米操作工具的进步，以及受限分子的理论处理，使我们能够更深入地了解这些特性。这项发现资助将使我的团队能够利用我们的单分子显微镜工具来探索有关蛋白质和 DNA 系统在纳米尺度上的行为的开放性问题；这些问题是其他方法无法解决的，并且具有国际研究兴趣和影响。