Microprocessor Controlled Liquid Chromatography System to Teach Principles of Chromatography and Protein Purification in a Core Quantitative Biology Course

9451989名斯威尼的学生培养批判性思维、分析数据、得出结论和做出决定的能力，这些能力涉及解决蛋白质分离相关问题的方法论的适当性。在利用自动化系统对一系列蓖麻毒素A蛋白(在结构上不同于1个或几个氨基酸)进行层析纯化的小组项目中，阐明了分子的物理/化学性质与其层析行为之间的关系。学生们还被介绍了基本的色谱学原理，因为它们被应用于蛋白质纯化。在三年级完成数量生物学的学生有资格参加暑期和/或四年级的本科生研究限制选修课。

9451989 Sweeney Students develop abilities to think critically, analyze data, draw conclusions and make decisions concerning the appropriateness of the methodology as applied to the solution of problems related to separation of proteins. During group projects utilizing the automated system to chromatographically purify a series of ricin A proteins (differing structurally in 1 or a few amino acids), the relationship between the physical/chemical nature of the molecules and their chromatographic behavior is elucidated. The students are also introduced to basic chromatographic principles as they are applied to protein purification. Students who complete Quantitative Biology during their junior year are qualified to participate in the undergraduate research restricted elective during summer and/or senior year.