Microprocessor Controlled Liquid Chromatography System to Teach Principles of Chromatography and Protein Purification in a Core Quantitative Biology Course

9451989斯威尼培养学生批判性思考、分析数据、得出结论和作出决定的能力，这些能力与应用于解决与蛋白质分离有关的问题的方法的适当性有关。在小组项目中，利用自动化系统对一系列蓖麻毒素a蛋白（在1个或几个氨基酸结构上不同）进行了层析纯化，阐明了分子的物理/化学性质与其层析行为之间的关系。本课程亦向学生介绍用于蛋白质纯化的基本色谱原理。在大三期间完成定量生物学的学生有资格参加暑期和/或大四的本科生研究限制选修课程。

9451989 Sweeney Students develop abilities to think critically, analyze data, draw conclusions and make decisions concerning the appropriateness of the methodology as applied to the solution of problems related to separation of proteins. During group projects utilizing the automated system to chromatographically purify a series of ricin A proteins (differing structurally in 1 or a few amino acids), the relationship between the physical/chemical nature of the molecules and their chromatographic behavior is elucidated. The students are also introduced to basic chromatographic principles as they are applied to protein purification. Students who complete Quantitative Biology during their junior year are qualified to participate in the undergraduate research restricted elective during summer and/or senior year.