Microprocessor Controlled Liquid Chromatography System to Teach Principles of Chromatography and Protein Purification in a Core Quantitative Biology Course

9451989 Sweeney学生培养批判性思考，分析数据，得出结论和做出关于适用于解决与蛋白质分离相关问题的方法的适当性的决定的能力。 在利用自动化系统色谱纯化一系列蓖麻毒素A蛋白（结构上有1个或几个氨基酸不同）的小组项目中，阐明了分子的物理/化学性质与其色谱行为之间的关系。学生还介绍了基本的色谱原理，因为它们适用于蛋白质纯化。 谁在大三完成定量生物学的学生有资格参加在夏季和/或高年级本科研究限制选修。

9451989 Sweeney Students develop abilities to think critically, analyze data, draw conclusions and make decisions concerning the appropriateness of the methodology as applied to the solution of problems related to separation of proteins. During group projects utilizing the automated system to chromatographically purify a series of ricin A proteins (differing structurally in 1 or a few amino acids), the relationship between the physical/chemical nature of the molecules and their chromatographic behavior is elucidated. The students are also introduced to basic chromatographic principles as they are applied to protein purification. Students who complete Quantitative Biology during their junior year are qualified to participate in the undergraduate research restricted elective during summer and/or senior year.