Innovation in the Undergraduate Chemistry Curriculum through Integration of FT-NMR Spectroscopy

9452027英国现代核磁共振原理和实践正在被整合到化学课程的低级和高级课程中。讲座和实验室的创新不仅影响到该系化学专业的学生，也影响到了注册生物和预科专业的学生。该部门正在将一台具有宽带和变温能力的200 MHz傅里叶变换核磁共振波谱仪与位于一座新设计的科学大楼内的新型实验室/计算机工作站环境中的个人计算机联网，以便进行实时光谱处理和讨论。课程的变化从大二的有机化学顺序开始：第一节课的早期介绍了核磁共振光谱学，并用宽带去耦合的13C谱和13C DEPT谱说明了异构体、命名、反应和反应机理的讲座讨论。通过HETCOR谱将~1H-核磁共振与~(13)C结合在一起。在有机实验室中，学生有机会生成其产品的13C和DEPT或HETCOR谱，这是对课堂中使用这些谱的补充。该仪器还可用于解释有机反应中的旋转异构化、立体选择性和区域选择性等现象。在大三的时候，无机化学专业的学生先合成一个配体并生成一个新的络合物，然后利用变温核磁共振来减缓可逆络合的快速交换动力学，并测定络合物的离解常数。生物化学序列的高年级学生获得并解释神经肽的COSY和NOESY谱，确定不同化学环境中的肽二级结构特征。在物理化学中，这些学生利用核磁共振观察溶剂对分子构象的影响，并对构象变化进行热力学分析。该仪器在定向研究(本科研究)课程中得到了更多的使用；无机、物理和生物化学的研究项目也可用。

9452027 Brittain Modern NMR principles and practice are being integrated into both lower- and upper-level courses in the chemistry curriculum. The innovations in the lecture and laboratory affect not only the department's chemistry majors, but also students enrolled in the biology and pre-professional programs. The department is networking a 200 MHz Fourier transform NMR spectrometer with broadband and variable temperature capabilities to personal computers located in a novel laboratory/computer-workstation environment in a newly designed science building to permit real-time spectral processing and discussion. The curricular changes begin in the sophomore organic chemistry sequence: NMR spectroscopy is being introduced early in the first course, and lecture discussions of isomerism, nomenclature, reactions and reaction mechanisms are illustrated with broadband-decoupled 13C spectra and 13C DEPT spectra. 1H-NMR is integrated with 13C through HETCOR spectroscopy. In the organic laboratory, the opportunity for students to generate 13C and DEPT or HETCOR spectra of their products complements the use of these spectra in lecture. The instrument also is used to illustrate phenomena such as rotational isomerism and stereo- and regioselectivity in organic reactions. In the junior year, students enrolled in Inorganic Chemistry synthesize a ligand and create a new complex, then utilize variable temperature NMR to slow the fast-exchange dynamics of reversible complexation and determine a complex dissociation constant. Senior students in the biochemistry sequence obtain and interpret COSY and NOESY spectra of neuropeptides, determining peptide secondary structural features in various chemical environments. In physical chemistry, these students utilize NMR to observe the effect of solvent on molecular conformation and perform a thermodynamic analysis of the conformational change. The instrument receives additional usage in directed studies (undergraduate research) cou rses; research projects in inorganic, physical, and biochemistry are available.