Improvement of Molecular Genetics Laboratories

分子遗传学实验室的改进

• 批准号: 9452306
• 负责人: Harry Wistrand
• 金额: $ 0.78万
• 依托单位: Agnes Scott College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1996-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9452306&HistoricalAwards=false
• 关键词: Improvement; Molecular; Genetics; Laboratories

9452306 Wistrand The proposal is for equipment and software to upgrade laboratories in the Molecular Genetics course (Biology 316) to incorporate recent advances in molecular genetics, especially in the area of DNA analysis. What were once highly specialized procedures in molecular biology have become standard; the polymerase chain reaction (PCR) is utilized as often as electrophoresis and has substituted in many cases for the CsCl DNA extraction technique for preparation of small amounts of DNA. This project requests funding to: 1) add the PCR protocols to the laboratory by acquiring a thermal cycler; and 2) add computer hardware and software to design PCR primers, to allow for entry of DNA sequences directly into the computer with the current sequencing gel reader, and analyze and compare sequences with those in databases such as GenBank. These additions to the laboratory will allow women science students to have direct experience with techniques that are becoming standard in research laboratories, graduate school, and medicine.

9452306 Wistrand建议使用设备和软件来升级分子遗传学课程（生物学316）的实验室，以结合分子遗传学的最新进展，特别是在DNA分析领域。分子生物学中曾经高度专业化的程序已经成为标准；聚合酶链反应（PCR）与电泳一样常用，在许多情况下已取代CsCl DNA提取技术制备少量DNA。该项目要求资金用于：1)通过购买热循环器将PCR方案添加到实验室；2)增加计算机硬件和软件来设计PCR引物，允许用现有测序凝胶读取器将DNA序列直接输入计算机，并与GenBank等数据库中的序列进行分析和比较。这些对实验室的补充将使理科生能够直接体验正在成为研究实验室、研究生院和医学标准的技术。