Expanding Laboratory Experiences in the Cell and Molecular Biology Curricula of Biology Majors

扩大生物学专业细胞和分子生物学课程的实验室经验

• 批准号: 9452386
• 负责人: Richard Kowles
• 金额: $ 1.28万
• 依托单位: Saint Mary's University of Minnesota
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1996-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9452386&HistoricalAwards=false
• 关键词: Expanding; Laboratory; Experiences; Cell; Molecular

9452386 Kowles Laboratory capabilities in upper division biology core courses and the undergraduate student research program have been improved and diversified by the acquisition of equipment for conducting experiments that require precisely controlled environments. The added instrumentation includes a control chamber, floor model incubator shaker, shaker water bath, thermocycler, DNA sequencing apparatus, and an electroporation system. Laboratory activities added to the curriculum include: bacteria transformation, plasmid preparation, DNA sequencing, gene amplification and transient expression, and RNase protection in molecular biology; studies in protein synthesis, protein isolation, and cellular metabolism in biochemistry; cellular ATP determination and the dynamics of cell proliferation in cell biology; and a number of qualitative and quantitative techniques for undergraduate research projects.

9452386 Kowles通过购置设备进行需要精确控制环境的实验，提高了高年级生物学核心课程和本科生研究项目的实验室能力，并使其多样化。增加的仪器包括一个控制室，地板模型孵化器摇床，摇床水浴，热循环器，DNA测序仪，和电穿孔系统。课程中增加的实验活动包括：细菌转化，质粒制备，DNA测序，基因扩增和瞬时表达，分子生物学中的RNase保护；生物化学中蛋白质合成、蛋白质分离和细胞代谢的研究细胞生物学中细胞ATP的测定与细胞增殖动力学；以及一些本科生研究项目的定性和定量技术。