RUI: The Cytokinetics of a Maternal Effect in the Developing Endosperm of Maize

在流式细胞仪技术产生的数据中，观察到了影响玉米发育中胚乳DNA内复制活性的母体效应。这个项目的总体目标是扩大关于胚乳发育中这些母体效应的信息。具体地说，将进行测试以确定：(1)在广泛的自交系之间的正反交中母体效应的程度；(2)由母体效应控制的精确特征(S)；以及(3)这些母体效应在胚乳发育过程中的细胞动力学中的作用。对胚乳的流式细胞术分析将从双列杂交模式中获得，双列杂交是在内复制活动的高峰期，在延长的发育期内以紧密的间隔进行的。BRD/URD/单抗技术将与体外耳培养系统结合使用，以确定母体效应对细胞周期时相和DNA复制率的作用。这些信息可能会带来关于DNA复制、细胞周期计时、基因组相互作用、甚至配合力和杂交活力的新想法。存在这样的可能性，即这些信息最终可能以预测性的方式用于杂交亲本的选择。

A maternal effect influencing DNA endoreduplication activity in the developing endosperm of maize has been observed in data generated by flow cytometric techniques. The overall objective of this project is to expand the information concerning these maternal effects in the developing endosperm. Specifically, tests will be made to determine: (1) the extent of the maternal effects in reciprocal crosses among a wide range of inbreds; (2) the precise characteristic(s) that are governed by the maternal effects; and (3) the role of these maternal effects in the cytokinetics of the developing endosperm. Flow cytometric analyses of endosperm will be obtained from a diallel pattern of parents and F1 reciprocal crosses at the peak of endoreduplication activity and at closely spaced intervals over an extended developmental period. Brd/Urd/monoclonal antibody techniques in conjunction with an in vitro ear culture system will be used to determine the role of maternal effects upon cell cycle phases and DNA replication rates. The information could lead to new ideas about DNA replication, cell cycle timing, genome interaction, and even combining ability and hybrid vigor. The possibility exists that such information might eventually be used in a predictive manner for the selection of parents for hybridization.